17
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Actinorhizal Signaling Molecules: Frankia Root Hair Deforming Factor Shares Properties With NIN Inducing Factor

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Actinorhizal plants are able to establish a symbiotic relationship with Frankia bacteria leading to the formation of root nodules. The symbiotic interaction starts with the exchange of symbiotic signals in the soil between the plant and the bacteria. This molecular dialog involves signaling molecules that are responsible for the specific recognition of the plant host and its endosymbiont. Here we studied two factors potentially involved in signaling between Frankia casuarinae and its actinorhizal host Casuarina glauca: (1) the Root Hair Deforming Factor (CgRHDF) detected using a test based on the characteristic deformation of C. glauca root hairs inoculated with F. casuarinae and (2) a NIN activating factor (CgNINA) which is able to activate the expression of CgNIN, a symbiotic gene expressed during preinfection stages of root hair development. We showed that CgRHDF and CgNINA corresponded to small thermoresistant molecules. Both factors were also hydrophilic and resistant to a chitinase digestion indicating structural differences from rhizobial Nod factors (NFs) or mycorrhizal Myc-LCOs. We also investigated the presence of CgNINA and CgRHDF in 16 Frankia strains representative of Frankia diversity. High levels of root hair deformation (RHD) and activation of Pro CgNIN were detected for Casuarina-infective strains from clade Ic and closely related strains from clade Ia unable to nodulate C. glauca. Lower levels were present for distantly related strains belonging to clade III. No CgRHDF or CgNINA could be detected for Frankia coriariae (Clade II) or for uninfective strains from clade IV.

          Related collections

          Most cited references52

          • Record: found
          • Abstract: found
          • Article: not found

          Speak, friend, and enter: signalling systems that promote beneficial symbiotic associations in plants.

          Plants associate with a wide range of microorganisms, with both detrimental and beneficial outcomes. Central to plant survival is the ability to recognize invading microorganisms and either limit their intrusion, in the case of pathogens, or promote the association, in the case of symbionts. To aid in this recognition process, elaborate communication and counter-communication systems have been established that determine the degree of ingress of the microorganism into the host plant. In this Review, I describe the common signalling processes used by plants during mutualistic interactions with microorganisms as diverse as arbuscular mycorrhizal fungi and rhizobial bacteria.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Plant recognition of symbiotic bacteria requires two LysM receptor-like kinases.

            Although most higher plants establish a symbiosis with arbuscular mycorrhizal fungi, symbiotic nitrogen fixation with rhizobia is a salient feature of legumes. Despite this host range difference, mycorrhizal and rhizobial invasion shares a common plant-specified genetic programme controlling the early host interaction. One feature distinguishing legumes is their ability to perceive rhizobial-specific signal molecules. We describe here two LysM-type serine/threonine receptor kinase genes, NFR1 and NFR5, enabling the model legume Lotus japonicus to recognize its bacterial microsymbiont Mesorhizobium loti. The extracellular domains of the two transmembrane kinases resemble LysM domains of peptidoglycan- and chitin-binding proteins, suggesting that they may be involved directly in perception of the rhizobial lipochitin-oligosaccharide signal. We show that NFR1 and NFR5 are required for the earliest physiological and cellular responses to this lipochitin-oligosaccharide signal, and demonstrate their role in the mechanism establishing susceptibility of the legume root for bacterial infection.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              A plant regulator controlling development of symbiotic root nodules.

              Symbiotic nitrogen-fixing root nodules on legumes are founded by root cortical cells that de-differentiate and restart cell division to establish nodule primordia. Bacterial microsymbionts invade these primordia through infection threads laid down by the plant and, after endocytosis, membrane-enclosed bacteroids occupy cells in the nitrogen-fixing tissue of functional nodules. The bacteria excrete lipochitin oligosaccharides, triggering a developmental process that is controlled by the plant and can be suppressed. Nodule inception initially relies on cell competence in a narrow infection zone located just behind the growing root tip. Older nodules then regulate the number of nodules on a root system by suppressing the development of nodule primordia. To identify the regulatory components that act early in nodule induction, we characterized a transposon-tagged Lotus japonicus mutant, nin (for nodule inception), arrested at the stage of bacterial recognition. We show that nin is required for the formation of infection threads and the initiation of primordia. NIN protein has regional similarity to transcription factors, and the predicted DNA-binding/dimerization domain identifies and typifies a consensus motif conserved in plant proteins with a function in nitrogen-controlled development.
                Bookmark

                Author and article information

                Contributors
                Journal
                Front Plant Sci
                Front Plant Sci
                Front. Plant Sci.
                Frontiers in Plant Science
                Frontiers Media S.A.
                1664-462X
                18 October 2018
                2018
                : 9
                : 1494
                Affiliations
                [1] 1Laboratoire Commun de Microbiologie, Institut de Recherche pour le Développement/Institut Sénégalais de Recherches Agricoles/Université Cheikh Anta Diop, Centre de Recherche de Bel Air , Dakar, Senegal
                [2] 2Laboratoire Mixte International Adaptation des Plantes et Microorganismes Associés Aux Stress Environnementaux, Centre de Recherche de Bel Air , Dakar, Senegal
                [3] 3Laboratoire Campus de Biotechnologies Végétales, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop , Dakar, Senegal
                [4] 4Laboratoire des Symbioses Tropicales et Méditerranéennes, Institut de Recherche pour le Développement/INRA/CIRAD, Université Montpellier/SupAgro , Montpellier, France
                [5] 5UMR 7245, Molécules de Communication et Adaptation des Microorganismes, Muséum National d’Histoire Naturelle, Centre National de la Recherche Scientifique, Sorbonne Universités , Paris, France
                [6] 6Laboratoire de Botanique et de Biodiversité Végétale, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop , Dakar, Senegal
                [7] 7UMR Diversité Adaptation et Développement des Plantes (DIADE), Institut de Recherche pour le Développement , Montpellier, France
                [8] 8Ecologie Microbienne, UMR 5557 CNRS, Université Lyon 1 , Villeurbanne, France
                [9] 9Institut National des Sciences Appliquées et de Technologie, Université Carthage , Tunis, Tunisia
                [10] 10Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire , Durham, NH, United States
                Author notes

                Edited by: Ulrike Mathesius, Australian National University, Australia

                Reviewed by: Dugald Reid, Aarhus University, Denmark; Ton Bisseling, Wageningen University & Research, Netherlands

                *Correspondence: Sergio Svistoonoff, sergio.svistoonoff@ 123456ird.fr

                This article was submitted to Plant Evolution and Development, a section of the journal Frontiers in Plant Science

                Article
                10.3389/fpls.2018.01494
                6201211
                ec667b30-5628-466f-a36f-6a772c0f0e0d
                Copyright © 2018 Cissoko, Hocher, Gherbi, Gully, Carré-Mlouka, Sane, Pignoly, Champion, Ngom, Pujic, Fournier, Gtari, Swanson, Pesce, Tisa, Sy and Svistoonoff.

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                History
                : 31 May 2018
                : 25 September 2018
                Page count
                Figures: 4, Tables: 1, Equations: 0, References: 62, Pages: 12, Words: 0
                Funding
                Funded by: U.S. Department of Agriculture 10.13039/100000199
                Award ID: NIFA 2015-67014-22849
                Funded by: Institut de Recherche pour le Développement 10.13039/100012947
                Categories
                Plant Science
                Original Research

                Plant science & Botany
                symbioses,nodulation factors,nodule inception,casuarina,alnus,discaria
                Plant science & Botany
                symbioses, nodulation factors, nodule inception, casuarina, alnus, discaria

                Comments

                Comment on this article