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      Biotin and biotin analogs in human urine: biotin accounts for only half of the total.

      1 , ,

      The Journal of nutrition

      Oxford University Press (OUP)

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          Abstract

          In studies using avidin-binding assays to measure the urinary excretion of biotin, biotin is sometimes assumed to be equal to the detectable avidin-binding substances present. High performance liquid chromatography was used to separate avidin-binding substances in human urine, and the chromatographic fractions were assayed for avidin-binding substances (biotin and biotin analogs) by a sensitive, specific assay based on binding of biotin to [125I]avidin. In a study of ten normal adults, substantial amounts of avidin-binding substances other than biotin were detected, two of which were bisnorbiotin and biotin sulfoxide. These biotin analogs were initially identified by their chromatographic properties, and identities were confirmed by chemical conversion. The presence of avidin-binding substances in addition to biotin may have confounded previous measurements of the urinary excretion of biotin using avidin-binding assays. Because bioassay methods for biotin often use organisms for which one or more of these biotin analogs are growth factors, measurements of biotin in urine using some bioassay methods are likely to overestimate the concentrations of biotin.

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          Author and article information

          Journal
          J. Nutr.
          The Journal of nutrition
          Oxford University Press (OUP)
          0022-3166
          0022-3166
          Nov 1993
          : 123
          : 11
          Affiliations
          [1 ] Department of Pediatrics, University of Arkansas for Medical Science/Arkansas Children's Hospital, Little Rock 72202-3591.
          Article
          10.1093/jn/123.11.1844
          8229299

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