1
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      In Well‐Treated Celiac Patients Low‐Level Mucosal Inflammation Predicts Response to 14‐day Gluten Challenge

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          In celiac disease (CeD), gluten activates adaptive immune cells that cause damage to the small intestinal mucosa. Histological evaluation of intestinal biopsies allows for grading of disease severity. CeD can effectively be treated with a life‐long gluten‐free diet. Gluten challenge of treated CeD patients is used to confirm diagnosis and to test drug efficacy in clinical trials, but patients respond with different magnitudes to the same gluten challenge. In this study of 19 well‐treated CeD patients, proteome analysis of total tissue or isolated epithelial cell compartment from formalin‐fixed paraffin embedded biopsies collected before and after 14‐day gluten challenge demonstrates that patients with strong mucosal response to challenge have signs of ongoing tissue inflammation already before challenge. This low‐level tissue inflammation at baseline is paralleled by increased gluten specific CD4+ T‐cell frequencies in the gut and presence of a low‐level blood inflammatory profile. Thus, apparently well‐treated CeD is frequently not entirely quiescent, with presence of low‐grade inflammation and antigluten immunity in the gut mucosa. Histology assessment alone appears insufficient to judge full recovery and gut mucosal healing of CeD patients. The findings raise a concern whether a seemingly proper gluten‐free diet is able to curb gut inflammation in all CeD patients.

          Abstract

          Spatially resolved tissue proteome analysis reveals low‐level inflammation and antigluten immunity in gut of some patients that are apparently well‐treated with gluten‐free diet. These patients develop strong mucosal response to short‐term gluten challenge.

          Related collections

          Author and article information

          Contributors
          jorunn.stamnas@medisin.uio.no
          l.m.sollid@medisin.uio.no
          Journal
          Adv Sci (Weinh)
          Adv Sci (Weinh)
          10.1002/(ISSN)2198-3844
          ADVS
          Advanced Science
          John Wiley and Sons Inc. (Hoboken )
          2198-3844
          04 January 2021
          February 2021
          : 8
          : 4 ( doiID: 10.1002/advs.v8.4 )
          Affiliations
          [ 1 ] K.G. Jebsen Coeliac Disease Research Centre University of Oslo Oslo Norway
          [ 2 ] Department of Immunology University of Oslo and Oslo University Hospital‐Rikshospitalet Oslo 0372 Norway
          [ 3 ] Department of Gastroenterology Oslo University Hospital‐Ullevål Oslo 0450 Norway
          [ 4 ] Department of Gastroenterology Oslo University Hospital‐Rikshospitalet Oslo 0372 Norway
          Author notes
          Article
          ADVS2293
          10.1002/advs.202003526
          7887593
          © 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH

          This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

          Page count
          Figures: 5, Tables: 0, Pages: 7, Words: 3502
          Product
          Funding
          Funded by: Stiftelsen Kristian Gerhard Jebsen , open-funder-registry 10.13039/100007793;
          Award ID: SKGJ‐MED‐017
          Funded by: South‐Eastern Norway Regional Health Authority
          Award ID: 2013046
          Funded by: South‐Eastern Norway Regional Health Authority
          Award ID: 2016113
          Categories
          Communication
          Communications
          Custom metadata
          2.0
          February 17, 2021
          Converter:WILEY_ML3GV2_TO_JATSPMC version:5.9.7 mode:remove_FC converted:17.02.2021

          Comments

          Comment on this article