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      An in vitro Assay for Corticotropin Releasing Factor(s) using Suspensions of Isolated Pituitary Cells

      ,

      Neuroendocrinology

      S. Karger AG

      CRF, ACTH, Adenohypophysis, Isolated cells

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          Abstract

          A technique employing isolated pituitary cells for the bioassay of CRF is described. The physiological integrity of these cells is indicated by their retention of ACTH against a large concentration gradient under control conditions; and by the release of ACTH in response to appropriate stimuli, through a process which is not only time-dependent, but also requires Ca<sup>++</sup>, and is inhibited by concentrations of corticosterone found in the plasma of intact rats. Evaluated as a bioassay, the technique is simple, convenient, sensitive and accurate. The evaluation of specificity must await the identification of authentic CRF and its availability for use as a standard.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1973
          1973
          19 March 2008
          : 12
          : 4-5
          : 236-248
          Affiliations
          Department of Physiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio
          Article
          122173 Neuroendocrinology 1973;12:236–248
          10.1159/000122173
          4354350
          © 1973 S. Karger AG, Basel

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          Page count
          Pages: 13
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