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      Evidence for Developmental Changes of Type IV Collagen in Glomerular Basement Membrane

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          Abstract

          Digestion of adult glomerular basement membrane (GBM) with collagenase releases a number of peptides of which the noncoUagenous region of type IV collagen is detected by antibodies from patients with anti-GBM nephritis. In our study, 17 of 19 sera reacting with GBM, in indirect immunofluorescence, with cryostat sections of adult kidneys were negative with cryostat sections of fetal kidneys. However, after digestion with collagenase, a similar pattern of peptides was released from both fetal and adult GBM. SDS-PAGE immunoblotting revealed comparable antibody binding of all 19 sera with the peptides from either fetal or adult GBM. These findings suggest conformational differences between type IV collagen in fetal and adult GBM or masking of the antigen by other GBM constituents which may shield the antigen.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1987
          1987
          05 December 2008
          : 45
          : 4
          : 302-305
          Affiliations
          aInstitute of Immunology and cDivision of Pediatric Nephrology, Department of Pediatrics, University of Heidelberg; bInstitute of Pathology, Vincentius Hospital, Karlsruhe, FRG
          Article
          184168 Nephron 1987;45:302–305
          10.1159/000184168
          2438564
          © 1987 S. Karger AG, Basel

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          Page count
          Pages: 4
          Categories
          Original Paper

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