Evidence for Developmental Changes of Type IV Collagen in Glomerular Basement Membrane

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Digestion of adult glomerular basement membrane (GBM) with collagenase releases a number of peptides of which the noncoUagenous region of type IV collagen is detected by antibodies from patients with anti-GBM nephritis. In our study, 17 of 19 sera reacting with GBM, in indirect immunofluorescence, with cryostat sections of adult kidneys were negative with cryostat sections of fetal kidneys. However, after digestion with collagenase, a similar pattern of peptides was released from both fetal and adult GBM. SDS-PAGE immunoblotting revealed comparable antibody binding of all 19 sera with the peptides from either fetal or adult GBM. These findings suggest conformational differences between type IV collagen in fetal and adult GBM or masking of the antigen by other GBM constituents which may shield the antigen.

Related collections

Author and article information

Journal

Journal ID (publisher-id): NEF

Journal ID (nlm-ta): Nephron

Journal ID (doi): 10.1159/issn.1660-8151

Title: Nephron

Publisher: S. Karger AG

ISSN (Print): 1660-8151

ISSN (Electronic): 2235-3186

Publication date Subscription-year: 1987

Publication date (Print): 1987

Publication date (Electronic): 05 December 2008

Volume: 45

Issue: 4

Pages: 302-305

Affiliations

^aInstitute of Immunology and ^cDivision of Pediatric Nephrology, Department of Pediatrics, University of Heidelberg; ^bInstitute of Pathology, Vincentius Hospital, Karlsruhe, FRG

Article

Publisher ID: 184168 Other ID: Nephron 1987;45:302–305

DOI: 10.1159/000184168

PubMed ID: 2438564

SO-VID: ecdebae3-dffc-46eb-a366-51a471fe7db9

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date accepted : 01 July 1986

Page count

Pages: 4

Comments

Comment on this article

scite_

Call for Papers: Green Renal Replacement Therapy: Caring for the Environment

Submit here before July 31, 2024