Blog
About

18
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Biotransformation of quercetin by Gliocladium deliquescens NRRL 1086

      Read this article at

      ScienceOpenPublisher
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          With an attempt to synthesize high-value isoquercitrin (quercetin-3- O-β-D-glucopyranoside), we carried out the biotransformation of quercetin ( 1) by Gliocladium deliquescens NRRL 1086. Along with the aimed product quercetin 3- O-β-D- glycoside ( 2), three additional metabolites, 2-protocatechuoyl-phlorogucinol carboxylic acid ( 3), 2,4,6-trihydroxybenzoic acid ( 4), and protocatechuic acid ( 5), were also isolated. The time-course experiments revealed that there were two metabolic routes, regio-selectivity glycosylation and quercetin 2,3-dioxygenation, co-existing in the culture. Both glycosylation and oxidative cleavage rapidly took place after quercetin feeding; about 98% quercetin were consumed within the initial 8 h and the oxdized product (2-protocatechuoyl-phlorogucinol carboxylic acid) was hydrolyzed into two phenolic compounds (2,4,6-trihydroxybenzoic acid and protocatechuic acid). We also investigated the impact of glucose content and metal ions on the two reactions and found that high concentrations of glucose significantly inhibited the oxidative cleavage and improved the yield of isoquercitrin and that Ca 2+, Fe 2+, Mn 2+, Mg 2+, and Zn 2+ inhibited glycosylation. To test the promiscuity of this culture, we selected other four flavonols as substrates; the results demonstrated its high regio-selectivity glycosylation ability towards flavonols at C-3 hydroxyl. In conclusion, our findings indicated that the versatile microbe of G. deliquescens NRRL 1086 maitained abundant enzymes, deserving further research.

          Related collections

          Author and article information

          Journal
          CJNM
          Chinese Journal of Natural Medicines
          Elsevier
          1875-5364
          20 August 2017
          : 15
          : 8
          : 615-624
          Affiliations
          1Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 210009, China
          2Institute of Biotechnology for TCM Research, School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 211198, China
          Author notes
          *Corresponding author: YU Bo-Yang, Tel: 86-25-83271321; Fax: 86-25-83313080, E-mail: boyangyu59@ 123456163.com ; ZHANG Jian, Tel: 86-25-86185157, Fax: 86-25-86185158, E-mail: wilson1978@ 123456163.com

          These authors have no conflict of interest to declare.

          Article
          S1875-5364(17)30089-4
          10.1016/S1875-5364(17)30089-4
          Copyright © 2017 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
          Funding
          Funded by: National Natural Science Foundation of China
          Award ID: 21302052
          This work was supported by the National Natural Science Foundation of China (No. 21302052).

          Comments

          Comment on this article