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      Predicted expansion of the claudin multigene family.

      Febs Letters
      Amino Acid Sequence, Animals, Cell Line, Claudins, chemistry, genetics, metabolism, Conserved Sequence, Databases, Protein, Dogs, Epithelial Cells, ultrastructure, Gene Expression Regulation, Developmental, Gene Library, Humans, Mice, Organ Specificity, Phylogeny, Protein Isoforms, Protein Structure, Secondary, RNA, Messenger, Recombinant Proteins, Sequence Alignment, Tight Junctions

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          Abstract

          Claudins (Cldn) are essential membrane proteins of tight junctions (TJs), which form the paracellular permselective barrier. They are produced by a multi-gene family of 24 reported members in mouse and human. Based on a comprehensive search combined with phylogenetic analyses, we identified three novel claudins (claudin-25, -26, and -27). Quantitative RT-PCR revealed that the three novel claudins were expressed in a tissue- and/or developmental stage-dependent manner. Claudins-25 and -26, but not claudin-27, were immunofluorescently localized to TJs when exogenously expressed in cultured MDCK and Eph epithelial cell lines. These findings expand the claudin family to include at least 27 members. Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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