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      An Efficient Method for the Isolation and Purification of Oligoribonucleotides

      , , , , , ,
      Nucleosides and Nucleotides
      Informa UK Limited

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          Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates.

          A method is described to synthesize small RNAs of defined length and sequence using T7 RNA polymerase and templates of synthetic DNA which contain the T7 promoter. Partially single stranded templates which are base paired only in the -17 to +1 promoter region are just as active in transcription as linear plasmid DNA. Runoff transcripts initiate at a unique, predictable position, but may have one nucleotide more or less on the 3' terminus. In addition to the full length products, the reactions also yield a large amount of smaller oligoribonucleotides in the range from 2 to 6 nucleotides which appear to be the result of abortive initiation events. Variants in the +1 to +6 region of the promoter are transcribed with reduced efficiency but increase the variety of RNAs which can be made. Transcription reaction conditions have been optimized to allow the synthesis of milligram amounts of virtually any RNA from 12 to 35 nucleotides in length.
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            Antisense oligonucleotides: a new therapeutic principle

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              Advances in the Synthesis of Oligonucleotides by the Phosphoramidite Approach

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                Author and article information

                Journal
                Nucleosides and Nucleotides
                Nucleosides and Nucleotides
                Informa UK Limited
                0732-8311
                February 1995
                February 1995
                : 14
                : 1-2
                : 255-273
                Article
                10.1080/15257779508014668
                ecf9ce3a-e7d4-40d2-b35d-c25348f26511
                © 1995
                History

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