9
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      Are you tired of sifting through news that doesn't interest you?
      Personalize your Karger newsletter today and get only the news that matters to you!

      Sign up

      • Record: found
      • Abstract: found
      • Article: found

      Effect of Sulfur Hexafluoride Gas on Antibacterial Activity of Antibiotics in vitro against Agents Causing Endophthalmitis

      research-article

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Background: We conducted a study to evaluate the effect of sulfur hexafluoride gas (SF<sub>6</sub>) on the antibacterial activity of antibiotics in vitro against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa, which are common endophthalmitis-causing agents. Methods: In this experimental study, antibiotic susceptibility tests were prepared according to the National Committee for Clinical Laboratory Standards macrobroth dilution method. Müller-Hinton broth was the test medium. Standard P. aeruginosa (ATCC 27853), S. epidermidis (ATCC 12228) and S. aureus (ATCC 29213) strains were studied. For P. aeruginosa ceftazidime, ofloxacin and tobramycin dilutions, and for S. epidermidis and S. aureus, cefazolin, ofloxacin and tobramycin dilutions were prepared identically in two sets of tubes. One set of tubes, into which pure SF<sub>6</sub> was injected, was defined as the SF<sub>6</sub> group. The other set of tubes, into which no SF<sub>6</sub> was injected, was taken as the control group. To determine the minimal bactericidal concentrations of the antibiotics, subcultures were made onto Müller-Hinton agar, and the colonies were counted after 18 h of incubation at 37°C. Results: The minimal inhibitory concentrations of the antibiotics in the SF<sub>6</sub> group were found to be identical with those of the control group. However, the minimal bactericidal concentrations of the antibiotics were found to be at least two dilutions lower in the SF<sub>6</sub> group than in the control group, except for the minimal bactericidal concentration of cefazolin for S. aureus, which was found to be one dilution lower in the SF<sub>6</sub> group. Conclusion: SF<sub>6</sub> was found to potentiate the in vitro antibacterial activity of ofloxacin and tobramycin against S. aureus, S. epidermidis and P. aeruginosa, ceftazidime against P. aeruginosa, and cefazolin against S. epidermidis. Experimental animal studies are required to determine the role of SF<sub>6</sub> in the management of endophthalmitis.

          Related collections

          Most cited references1

          • Record: found
          • Abstract: not found
          • Article: not found

          Evaluation of toxicity of intravitreal ceftazidime, vancomycin, and ganciclovir in a silicone oil-filled eye

            Bookmark

            Author and article information

            Journal
            OPH
            Ophthalmologica
            10.1159/issn.0030-3755
            Ophthalmologica
            S. Karger AG
            0030-3755
            1423-0267
            2001
            December 2001
            30 November 2001
            : 215
            : 6
            : 439-443
            Affiliations
            Departments of aOphthalmology and bClinical Bacteriology and Infection Diseases, Kocaeli University, School of Medicine, Izmit, Turkey
            Article
            50905 Ophthalmologica 2001;215:439–443
            10.1159/000050905
            11741112
            ed4ded18-4f6b-4ae3-a3c3-008c420e74bb
            © 2001 S. Karger AG, Basel

            Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

            History
            Page count
            Tables: 2, References: 13, Pages: 5
            Categories
            Original Paper · Travail original · Originalarbeit

            Vision sciences,Ophthalmology & Optometry,Pathology
            Endophthalmitis,Antibiotics,Sulfur hexafluoride gas

            Comments

            Comment on this article