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      Experiments on Corneal Preservation (Short-Time Storage)

      , ,
      Ophthalmic Research
      S. Karger AG
      Moist chamber, M-K medium, Corneal storage, Short-term, Cornea, Viability test

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          56 human eyes were stored in a moist chamber from 0 to 4 days at +4°C. 20 human corneal buttons with a scleral rim were stored in the M-K medium for 5 and 7 days at +4°C. After this procedure in both series the temperature reversal effect was measured and the living endothelial cells were counted after being stained with trypan blue and rose bengal. All eyes showed a typical swelling-shrinking phenomena but after 4 days in a moist chamber this effect was definitely less characteristic than in all other periods of storage time. In respect to the endothelial cell counts 5 day M-K medium storage corresponds to 2 day moist chamber storage, and 7 day M-K storage is comparable to 3 day moist chamber storage. The total survival time of the endothelial cells in both series is almost equal.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          03 December 2009
          : 8
          : 2
          : 124-132
          Section Microsurgery of the Eye, Eye Clinic, University of Bonn, Bonn
          264807 Ophthalmic Res 1976;8:124–132
          © 1976 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          : 23 January 1975
          : 04 September 1975
          Page count
          Pages: 9

          Vision sciences,Ophthalmology & Optometry,Pathology
          Viability test,Cornea,Moist chamber,Corneal storage,M-K medium,Short-term


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