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      Influence of Interleukin 10 and Transforming Growth Factor-β on T Cell Stimulation through Allogeneic Retinal Pigment Epithelium Cells in vitro

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          Abstract

          The effect of interleukin 10 (IL-10) and transforming growth factor-β (TGF-β) on the immunological capability of human retinal pigment epithelium (RPE) cells was tested by measuring the proliferation of allogeneic T cells after stimulation through the RPE cells in vitro. For this, human RPE cells were activated with different concentrations of interferon-γ (0, 50, 100 and 250 U/ml) used as stimulator cells. The following T cell proliferation was measured with a tetrazolium-based colorimetric assay over a period of up to 4 days. Our results show that RPE cells are able to stimulate allogeneic T cells in the presence or absence of professional antigen-presenting cells (macrophages). Additionally, the proliferation could be significantly decreased by IL-10 or TGF-β. The maximum of suppression, especially by TGF-β, was found after 48 and 72 h of incubation. This shows that the antigen-presenting properties of human RPE cells can be altered in culture.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          2002
          August 2002
          16 September 2002
          : 34
          : 4
          : 232-240
          Affiliations
          aDepartment of Ophthalmology and Visual Sciences, School of Medicine, University of Louisville, Louisville, Ky., USA; bDepartment of Ophthalmology, Faculty of Medicine, University of Leipzig, Leipzig, Germany
          Article
          63875 Ophthalmic Res 2002;34:232–240
          10.1159/000063875
          12297696
          ed645300-62f0-4c08-b471-1a98b74aef03
          © 2002 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 18 May 2001
          : 20 December 2001
          Page count
          Figures: 7, Tables: 1, References: 28, Pages: 9
          Categories
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          T cell proliferation,Interleukin 10,Transforming growth factor-β,Interferon-=γ,Retinal pigment epithelium

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