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      An investigation of the possible methods and potential benefits of de novo cloning of Nannochloropsis oceanica genes

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          Abstract

          Species in the microalgal genus Nannochloropsis are increasingly used as models for theoretical and applied studies. Here we attempt to generate InDel variations in the genome of Nannochloropsis oceanica, and then decipher the genetic basis of its economic and biological traits with bulked mutant analysis modified from bulked segregant analysis. In addition, we describe our efforts to construct site-tagged and gene-traceable mutant libraries to clone its genes through reverse genetic approaches. Currently, more than a half of N. oceanica protein-encoding genes are annotated against databanks. However, no functional gene has been de novo cloned from N. oceanica and no new function has been assigned to any of its annotatable genes. Here, we discuss the possible methods and potential benefits of de novo cloning of N. oceanica genes.

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          Author and article information

          Journal
          MLST
          Marine Life Science & Technology
          Springer (China )
          2096-6490
          2662-1746
          01 November 2019
          02 December 2019
          : 1
          : 1
          : 22-27
          Affiliations
          1College of Marine Life Sciences, Ocean University of China (OUC), Qingdao 266003, China
          2Institutes of Evolution and Marine Biodiversity, OUC, Qingdao 266003, China
          3Key Laboratory of Marine Genetics and Breeding of Ministry of Education, OUC, Qingdao 266003, China
          Author notes
          *Corresponding author: Li Guo, E-mail: qdguoli@ 123456139.com
          Article
          s42995-019-00014-1
          10.1007/s42995-019-00014-1
          © 2019 Ocean University of China
          Product
          Self URI (journal-page): https://www.springer.com/journal/42995
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