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      Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo

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          Abstract

          Purpose

          We attempted to develop a device for measuring electrical corneal resistance (CR) using corneal contact lens electrodes to quantitatively evaluate corneal injury in vivo. In the present study, full-thickness detachment of the corneal epithelium was induced by n-heptanol, and the feasibility of the quantitative evaluation of this injury by corneal contact lens electrodes was evaluated in vivo.

          Methods

          The central area of an albino rabbit’s cornea was exposed to a filter paper pre-immersed in n-heptanol for 1 minute to induce injury of the corneal epithelium. After induction of injury, the electrical CR was measured and the percentage of CR (%CR) was calculated. Fluorescein solution (3 μL) was applied to the wound/affected area of the corneal epithelium for photography with a slit-lamp biomicroscope. The wound/affected area was measured using an image analysis system. The correlation between the %CR and the wound/affected area was analyzed.

          Results

          As the size of the wound/affected area of the corneal epithelium increased, the %CR decreased after corneal epithelium detachment. Thus, a close correlation was found between the area of corneal epithelium detachment and the %CR.

          Conclusion

          The corneal resistance device that we developed was capable of quantitatively evaluating n-heptanol-induced full-thickness injuries of the corneal epithelium.

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          Author and article information

          Journal
          Clin Ophthalmol
          Clin Ophthalmol
          Clinical Ophthalmology
          Clinical Ophthalmology (Auckland, N.Z.)
          Dove Medical Press
          1177-5467
          1177-5483
          2012
          2012
          17 April 2012
          : 6
          : 585-593
          Affiliations
          [1 ]Department of Ophthalmology, Kanazawa Medical University, Uchinada, Japan
          Author notes
          Correspondence: Masamichi Fukuda, Department of Ophthalmology, Kanazawa Medical University, Daigaku 1-1, Uchinada-machi, Kahoku-gun, Ishikawa 920-0293, Japan, Tel +81 76 286 2211 ext 3417, Fax +81 76 286 1010, Email fukuda@ 123456kanazawa-med.ac.jp
          Article
          opth-6-585
          10.2147/OPTH.S30935
          3340121
          22553418
          ee284d3d-32fa-469e-abcb-48fa106fddee
          © 2012 Fukuda and Sasaki, publisher and licensee Dove Medical Press Ltd.

          This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.

          History
          Categories
          Original Research

          Ophthalmology & Optometry
          corneal contact lens electrode corneal resistance device,n-heptanol,eyes in vivo,corneal injury

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