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      A formula for scoring human embryo growth rates in in vitro fertilization: Its value in predicting pregnancy and in comparison with visual estimates of embryo quality

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      Journal of In Vitro Fertilization and Embryo Transfer
      Springer Nature

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          Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo

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            A method for collecting motile spermatozoa from human semen.

            Spermatozoa migrate rapidly into a diluent brought into contact with semen. When this process was used to collect sperm in a diluent contained in Pasteur pipettes that were dipped into semen for 1 hour, concentrations of spermatozoa ranging from 0.6 to 5.7 X 10(6) cells/ml were obtained. This method yielded sperm suspensions free of seminal plasma and populations of spermatozoa that had increased motility ratings compared with the original semen. In contrast, removal of the seminal plasma by a procedure involving three dilutions and two gentle centrifugations resulted in a decrease in the average motility ratings. Determinations were made of the electrolyte, energy substrate, and trace element content of preovulatory human tubal fluid. A sperm diluent having an electrolyte composition similar to that of tubal fluid was formulated and used for collecting spermatozoa. Population of spermatozoa that migrated from semen into this diluent had significantly greater mean velocities than sperm in the semen. Moreover, the diluent proved to be a favourable medium for significantly delaying the deterioration of sperm progression over a 24-hour period.
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              The technique for human embryo transfer.

              In a program of human in vitro fertilization (IVF), the results of 204 attempted intracervical embryo transfers (ETs), using a variety of catheters in three trials over 18 months, have been analyzed for the ease of transfer and pregnancy rate. In nulliparous patients, transfers were more difficult than in multiparous patients; and a closed-end Teflon catheter was found to be more easily passed through the smaller cervical canal than an open-end catheter. The overall pregnancy rate was 17% (March 1980 to August 1981) and was not related to catheter type, although when chemical pregnancies were excluded, it was found that transfers using open-end catheters were more successful. The transfer procedure developed finally for routine use incorporates a consideration of these results.
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                Author and article information

                Journal
                Journal of In Vitro Fertilization and Embryo Transfer
                J Assist Reprod Genet
                Springer Nature
                0740-7769
                1573-7330
                October 1986
                October 1986
                : 3
                : 5
                : 284-295
                Article
                10.1007/BF01133388
                3783014
                ee29e4d1-1573-4bf4-b532-5b4bddd49fe7
                © 1986
                History

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