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      Análise de proteínas e isoenzimas de isolados de Rhizoctonia spp. patogênicos a Eucalyptus Translated title: Protein and isozyme analysis of isolates of Rhizoctonia spp. pathogenic to Eucalyptus

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          Abstract

          Objetivou-se caracterizar isolados de Rhizoctonia solani AG1 e AG4 e isolados binucleados de Rhizoctonia spp. patogênicos a Eucalyptus, por meio de eletroforese de proteínas, em gel de poliacrilamida, e de isoenzimas (ACP, 6-PGDH, LAP, SOD, MDH e IDH), em gel de amido. Para comparação, incluíram-se alguns isolados brasileiros de outros hospedeiros e isolados-padrões de R. solani AG1, procedentes do Japão. Observaram-se diferenças nos padrões gerais de proteínas e nos fenótipos isoenzimáticos entre isolados binucleados e multinucleados e entre isolados de diferentes grupos e subgrupos de anastomose. Isolados de R. solani AG1, procedentes do Brasil e Japão, apresentaram baixa similaridade nos padrões de proteínas e de isoenzimas. Isolados brasileiros morfologicamente semelhantes a R. solani AG1-IB (microesclerodiais) apresentaram padrões de proteínas similares e um maior número de fenótipos isoenzimáticos idênticos entre si. Esta tendência foi independente do hospedeiro e da origem geográfica. Variações nos padrões de proteínas e de isoenzimas foram também observadas dentre isolados brasileiros de R. solani AG4. Discute-se o uso da eletroforese de proteínas e isoenzimas na caracterização de isolados de Rhizoctonia spp. e em estudos genéticos e filogenéticos de fungos deste gênero.

          Translated abstract

          Isolates of Rhizoctonia solani (anastomosis groups AG1 and AG4) and binucleate isolates of other unidentified species, all pathogenic to Eucalyptus in Brazil, were characterized by protein and isozyme (ACP, 6-PGDH, LAP, SOD, MDH e IDH) analysis. Japanese strains of R. solani AG1 and other Brazilian isolates from others hosts were also included for comparison. Differences in protein patterns and isozyme phenotypes were observed between binucleate and multinucleate isolates, and among isolates of different anastomosis groups and subgroups. The protein patterns and isozyme phenotypes of Japanese isolates of R. solani AG1 differed from those of Brazilian isolates of the same anastomosis groups and the same morphological subgroups. Some Brazilian isolates morphologically similar to R. solani AG1-IB presented high similarity in protein patterns and isozyme phenotypes. This similarity was independent of the host plant and geographical origin. Polymorphisms in protein and isozyme patterns were also observed within the Brazilian R. solani AG4 group. We discuss the usefulness of protein and isozyme analyses for characterization of Rhizoctonia spp. isolates and for genetic and phylogenetic studies of fungi of this genus.

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          Most cited references 22

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          Ecology and Pathogenicity of Anastomosis and Intraspecific Groups of Rhizoctonia Solani Kuhn

           A Ogoshi (1987)
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            Epidemiologia da mancha foliar da macieira

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              Ecology And Pathogenicity Of Anastomosis And Intraspecific Groups Of Rhizoctonia-Solani Khn

               A Ogoshi (1987)
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Journal
                fb
                Fitopatologia Brasileira
                Fitopatol. bras.
                Sociedade Brasileira de Fitopatologia (Brasília )
                1678-4677
                February 2002
                : 27
                : 1
                : 33-41
                Affiliations
                [1 ] Universidade Estadual do Norte Fluminense Brazil
                [2 ] Universidade Federal de Viçosa Brazil
                Article
                S0100-41582002000100005
                10.1590/S0100-41582002000100005
                Product
                Product Information: website
                Categories
                AGRONOMY
                PLANT SCIENCES

                Plant science & Botany, Horticulture

                Rhizoctonia solani, gel de amido, eletroforese de proteínas

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