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      Spectroscopic Methods Used in Implant Material Studies

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          Abstract

          It is recognized that interactions between most materials are governed by their surface properties and manifest themselves at the interface formed between them. To gain more insight into this thin layer, several methods have been deployed. Among them, spectroscopic methods have been thoroughly evaluated. Due to their exceptional sensitivity, data acquisition speed, and broad material tolerance they have been proven to be invaluable tools for surface analysis, used by scientists in many fields, for example, implant studies. Today, in modern medicine the use of implants is considered standard practice. The past two decades of constant development has established the importance of implants in dentistry, orthopedics, as well as extended their applications to other areas such as aesthetic medicine. Fundamental to the success of implants is the knowledge of the biological processes involved in interactions between an implant and its host tissue, which are directly connected to the type of implant material and its surface properties. This review aims to demonstrate the broad applications of spectroscopic methods in implant material studies, particularly discussing hard implants, surface composition studies, and surface–cell interactions.

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          Most cited references138

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          Fourier Transform Infrared (FTIR) Spectroscopy of Biological Tissues

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            Synthesis and applications of Rhodamine derivatives as fluorescent probes.

            Rhodamine dyes are widely used as fluorescent probes owing to their high absorption coefficient and broad fluorescence in the visible region of electromagnetic spectrum, high fluorescence quantum yield and photostability. A great interest in the development of new synthetic procedures for preparation of Rhodamine derivatives has arisen in recent years because for most applications the probe must be covalently linked to another (bio)molecule or surface. In this critical review the strategies for modification of Rhodamine dyes and a discussion on the variety of applications of these new derivatives as fluorescent probes are given (108 references).
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              Total internal reflection fluorescence microscopy in cell biology.

              Key events in cellular trafficking occur at the cell surface, and it is desirable to visualize these events without interference from other regions deeper within. This review describes a microscopy technique based on total internal reflection fluorescence which is well suited for optical sectioning at cell-substrate regions with an unusually thin region of fluorescence excitation. The technique has many other applications as well, most notably for studying biochemical kinetics and single biomolecule dynamics at surfaces. A brief summary of these applications is provided, followed by presentations of the physical basis for the technique and the various ways to implement total internal reflection fluorescence in a standard fluorescence microscope.
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                Author and article information

                Contributors
                Role: Academic Editor
                Journal
                Molecules
                Molecules
                molecules
                Molecules
                MDPI
                1420-3049
                29 January 2020
                February 2020
                : 25
                : 3
                : 579
                Affiliations
                Department of Biomedical Chemistry, Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland; przemyslaw.jurczak@ 123456ug.edu.pl (P.J.); natalia.karska@ 123456ug.edu.pl (N.K.); agnieszka.szczepanik@ 123456phdstud.ug.edu.pl (A.K.); aneta.szymanska@ 123456ug.edu.pl (A.S.)
                Author notes
                [* ]Correspondence: slawomir.lach@ 123456ug.edu.pl (S.L.); s.rodziewicz-motowidlo@ 123456ug.edu.pl (S.R.-M.); Tel.: +48-58-523-5034 (S.L.); +48-58-523-5037 (S.R.-M.)
                [†]

                These authors contributed equally to this work.

                Author information
                https://orcid.org/0000-0002-3985-2214
                https://orcid.org/0000-0001-7962-3953
                https://orcid.org/0000-0002-4471-5951
                Article
                molecules-25-00579
                10.3390/molecules25030579
                7038083
                32013172
                ee38dad0-f423-4078-9a03-d1673fae2ad2
                © 2020 by the authors.

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 01 January 2020
                : 25 January 2020
                Categories
                Review

                auger electron spectroscopy,x-ray photoelectron spectroscopy,raman spectroscopy,photoluminescence piezospectroscopy,fluorescence microscopy,methods,implant,surface

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