Three organic compounds of different hydrophobicity, 1,2,4,5-tetrachlorobenzene (TeCB), 3,4,5,6-tetrachloroguaiacol (TeCG) and 4,6-dichlorobenzenediol (DBD), were chosen as the test chemicals to carry out a series of investigations to look at the relationship between toxicant transfer and fish metabolic rate. A significant correlation was found between the toxicant uptake rate constant (k(1)) and fish oxygen consumption, regardless of fish size and species. This correlation was improved when fish toxicant body load was expressed on a percent body lipid basis. Similarly, there also existed a significant relationship between the toxicant depuration rate constant (k(2)) and fish oxygen uptake for a range of chemicals with different octanol/water partition coefficients (K(ow)).