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      Comparison of test results of Schistosomiasis cercariae from two different animal membranes by quantitative fluorescence PCR

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          Abstract

          Objective To compare influence of two kinds of animal membrane on obtaining Schistosoma cercariae, aiming at providing reference for optimal selection of animal membranes.

          Methods Two kinds of animal membrane, namely membrane with sodium benzoate and without sodium benzoate (hereinafter referred to as membrane A and membrane B) were prepared, while 3 groups were set according to the number of cercariae (1-10, 11-99, ≥100 cercariae). Quantitative fluorescent PCR (QF-PCR) was used for comprehensive comparison of influence on cercariae acquisition between two kinds of membrane.

          Results Laboratory test results showed that detection rates of Schistosoma cercariae from membrane A and membrane B were both 77.78% in the group of 1-10 cercariae and both 100% in the other two groups. The total detection rates were both 90.48%. There was no statistical significance observed in the comparison of detection rate of Schistosoma cercariae from membrane A and membrane B ( P>0.05). Besides, detection rates of Schistosoma cercariae from simulated on-the-spot membrane A and B were both 5.00%, showing no statistical significance either ( P>0.05).

          Conclusion There is no difference shown in influence on cercariae acquisition between membrane with and without sodium benzoate. As sodium benzoate can also play the role of preservative, it can be used in long-term preservation of animal membranes. Thus, bionic membranes containing sodium benzoate are suggested for continuous use on site.

          Abstract

          摘要: 目的 比较两种不同动物皮膜获取血吸虫尾蚴效果, 为动物皮膜优选提供参考。 方法 分别制作添加苯甲酸钠 (A膜) 与未添加苯甲酸钠 (B膜) 两种动物皮膜, 将尾蚴设置成1~10条、11~99条、≥100条3个浓度组, 采用荧光定量PCR法, 对两种动物皮膜获取尾蚴效果进行综合比较。 结果 实验室测试结果显示, 尾数1~10条组, A膜和B膜的血吸虫尾蚴检出率均为77.78%, 其余两个组检出率均为100%, 总检出率均为90.48%。A膜和B膜的血吸虫尾蚴检出率比较差异无统计学意义 ( P>0.05) 。对模拟现场的A膜和B膜的血吸虫尾蚴的检出率均为5.00%, 两者比较差异无统计学意义 ( P>0.05) 。 结论 添加与未添加苯甲酸钠动物皮膜获取尾蚴效果一致, 但因苯甲酸钠具有防腐剂作用, 可用于动物皮膜的长时间保存, 因此推荐含有苯甲酸钠的仿生膜用于现场连续使用。

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          Author and article information

          Journal
          CTM
          China Tropical Medicine
          China Tropical Medicine (China )
          1009-9727
          01 July 2020
          01 July 2020
          : 20
          : 7
          : 617-620
          Affiliations
          1The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Provincal Key Laboratory of Animal-origin and Vector-borne Diseases, Nanchang Center for Disease Control and Prevention. Nanchang, Jiangxi 330038, China
          2Shenzhen Combined Biotech Co., Ltd., Shenzhen, Guangdong 518057, China
          3Nanchang Xinjian District Schistosomiasis Control Station, Nanchang, Jiangxi 330100, China
          4National Institute of Parasitic Diseases, China CDC, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China
          Author notes
          Corresponding authors: HU Shaoliang, E-mail: cbd@ 123456biocbd.com ; YU Qing, E-mail: yuqing@ 123456nipd.chinacdc.cn
          Article
          j.cnki.46-1064/r.2020.07.06
          10.13604/j.cnki.46-1064/r.2020.07.06
          © 2020 Editorial Department of China Tropical Medicine

          This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 Unported License (CC BY-NC 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. See https://creativecommons.org/licenses/by-nc/4.0/.

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