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      The proprotein convertase furin in human trophoblast: Possible role in promoting trophoblast cell migration and invasion

      , , , , , , ,
      Placenta
      Elsevier BV

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          Abstract

          Furin, a proprotein convertase (PC), is ubiquitously expressed and implicated in many physiological and pathological processes. This study is aimed to identify the role of furin in human trophoblast invasion and migration. Furin was found to be highly expressed in placental villi of both rhesus monkeys and human beings during early pregnancy. Specifically, furin was found in trophoblast column and trophoblast shell, regions where highly invasive cytotrophoblast cells invade the maternal decidua during human placentation. To determine whether furin plays any role in trophoblast invasion and migration, we employed human extravillous HTR8/SVneo cells in Matrigel invasion and transwell migration assays. Knocking-down furin expression by siRNA significantly inhibited invasion and migration of HTR8/SVneo cells (P<0.01), with corresponding decrease of matrix metalloproteinase-9 (MMP-9) activities. In contrast, over-expression of furin markedly increased cell invasion and migration (P<0.01), accompanied by significant increase of MMP-9 activities. Furthermore, furin siRNA significantly increased the levels of both tissue inhibitors of MMPs (TIMP)-1 and -2. Our results suggest that furin may play an important role in the invasion and migration of human trophoblast cells during early pregnancy.

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          Author and article information

          Journal
          Placenta
          Placenta
          Elsevier BV
          01434004
          November 2009
          November 2009
          : 30
          : 11
          : 929-938
          Article
          10.1016/j.placenta.2009.09.003
          19853298
          ee8ddc9a-7821-4d1c-9c91-4656bcc1b018
          © 2009

          https://www.elsevier.com/tdm/userlicense/1.0/

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