Objective To explore the effect of allicin on learning ability, memory and antioxidant abilities of aging mice induced by d-galactose.
Methods A total of 50 healthy male mice were randomly divided into 5 groups ( n=10): normal control group (C), model control group (D), low-dose allicin group (10 mg/kg, L), medium-dose allicin group (20 mg/kg, M), and high-dose allicin group (40 mg/kg, H). After feeding for one week, the allicin groups and the model group were intraperitoneally injected with d-galactose normal saline solution (100 mg/kg), while the normal control group with normal saline of the same volume. Allicin groups was given different doses of allicin, and the same volume of normal saline was given to normal control group and model group for 42 d. Then the learning ability and memory of mice were measured by Morris water maze test, and the total antioxidant capacity in serum, the activities of SOD, GSH-Px, CAT and the content in serum, liver and brain tissues were detected.
Results Compared with the model group, the incubation period in the allicin group was significantly shorten, the number of times in the 120 s through the platform and the time after the original platform quadrant ratio were significantly increased ( P<0.05 or P<0.01). The total antioxidant capacity in serum of mice was significantly increased, the activities of SOD, GSH-Px and CAT in serum, brain and liver tissues were increased, and MDA content was reduced, all of which were statistically significant ( P<0.05 or P<0.01).
Conclusion Allicin can improve the learning and memory ability of d-galactose-induced aging mice, and its mechanism might be related to increasing antioxidant enzyme activity, scavenging free radicals and improving antioxidant ability.
摘要： 目的 探索大蒜素对D-半乳糖致衰老小鼠学习记忆及抗氧化能力的影响。 方法健康雄性小白鼠50只,按数字表法随机分为5组( n=10):正常对照组(C组)、模型对照组(D组)、大蒜素低剂量组（10 mg/kg,L组)、大蒜素中剂量组(20 mg/kg,M组)、大蒜素高剂量组(40 mg/kg,H组)。饲养1周后，大蒜素组和模型组腹腔注射D-半乳糖生理盐水溶液（100 mg/kg),正常对照组腹腔注射同体积生理盐水;大蒜素组灌胃不同剂量的大蒜素，正常对照组和模型组灌胃同体积的生理盐水，持续42 d。实验结束后，采用Morris水迷宫检测小鼠学习记忆能力，处死小鼠测定小鼠血清T-AOC,血清、肝、脑组织中的SOD、GSH-Px、CAT活性和MDA含量。 结果与模型组相比，大蒜素各剂量组逃避潜伏期时间明显缩短,120 s内穿过平台的次数和经过原平台象限的时间比率都显著增多( P<0.05或 P<0.01),小鼠血清中T-AOC显著升高，血清、脑、肝组织中SOD、GSH-Px、CAT活性均显著升高,MDA含量显著降低，差异均有统计学意义( P<0.05或 P<0.01)。 结论大蒜素能改善D-半乳糖致衰老小鼠的学习记忆能力，其机制可能与增加抗氧化酶活性，清除自由基、提高抗氧化能力有关。