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Identification and characterization of a myeloid differentiation factor 88 (MyD88) cDNA and gene in Japanese flounder, Paralichthys olivaceus.

Developmental and Comparative Immunology

microbiology, Adaptor Proteins, Signal Transducing, immunology, Spleen, Sequence Alignment, veterinary, Reverse Transcriptase Polymerase Chain Reaction, genetics, biosynthesis, RNA, Messenger, Myeloid Differentiation Factor 88, Molecular Sequence Data, Kidney, Immunohistochemistry, Flounder, Fish Diseases, Enterobacteriaceae Infections, Edwardsiella tarda, Blotting, Western, Base Sequence, Animals, Amino Acid Sequence

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      The interleukin-1 receptor/toll-like receptor (IL-1R/TLR) superfamily signaling involves myeloid differentiation factor 88 (MyD88) that acts as an important adapter protein. A Japanese flounder (Paralichthys olivaceus) MyD88 (jfMyD88) cDNA and gene were cloned, and found to have lengths of 1.5 and 3.01 kb, respectively. The ORF encodes 285 amino acids that contain a death domain and a Toll/IL-1 receptor domain. The gene is composed of 5 exons and 4 introns. The jfMyD88 gene is highly expressed in organs involved in immune functions, including the gills, intestines, kidney, skin and spleen. Three days after a fish was infected with Edwardsiella tarda, staining with anti-jfMyD88 polyclonal antibody revealed an increased population of MyD88-positive cells in the kidney and spleen. These results imply that MyD88 has an important role in the innate immune system in Japanese flounder.

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