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Association of Ferredoxin:NADP+ oxidoreductase with the photosynthetic apparatus modulates electron transfer in Chlamydomonas reinhardtii

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      Abstract

      Ferredoxins (FDX) and the FDX:NADP + oxidoreductase (FNR) represent a key junction of electron transport downstream of photosystem I (PSI). Dynamic recruitment of FNR to the thylakoid membrane has been considered as a potential mechanism to define the fate of photosynthetically derived electrons. In this study, we investigated the functional importance of the association of FNR with the photosynthetic apparatus in Chlamydomonas reinhardtii. In vitro assays based on NADP + photoreduction measurements as well as NMR chemical shift perturbation analyses showed that FNR preferentially interacts with FDX1 compared to FDX2. Notably, binding of FNR to a PSI supercomplex further enhanced this preference for FDX1 over FDX2, suggesting that FNR is potentially capable of channelling electrons towards distinct routes. NADP + photoreduction assays and immunoblotting revealed that the association of FNR with the thylakoid membrane including the PSI supercomplex is impaired in the absence of Proton Gradient Regulation 5 (PGR5) and/or Proton Gradient Regulation 5-Like photosynthetic phenotype 1 (PGRL1), implying that both proteins, directly or indirectly, contribute to the recruitment of FNR to the thylakoid membrane. As assessed via in vivo absorption spectroscopy and immunoblotting, PSI was the primary target of photodamage in response to high-light stress in the absence of PGR5 and/or PGRL1. Anoxia preserved the activity of PSI, pointing to enhanced electron donation to O 2 as the source of the observed PSI inactivation and degradation. These findings establish another perspective on PGR5/PGRL1 knockout-related phenotypes and potentially interconnect FNR with the regulation of photosynthetic electron transport and PSI photoprotection in C. reinhardtii.

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      PGR5 is involved in cyclic electron flow around photosystem I and is essential for photoprotection in Arabidopsis.

      During photosynthesis, plants must control the utilization of light energy in order to avoid photoinhibition. We isolated an Arabidopsis mutant, pgr5 (proton gradient regulation), in which downregulation of photosystem II photochemistry in response to intense light was impaired. PGR5 encodes a novel thylakoid membrane protein that is involved in the transfer of electrons from ferredoxin to plastoquinone. This alternative electron transfer pathway, whose molecular identity has long been unclear, is known to function in vivo in cyclic electron flow around photosystem I. We propose that the PGR5 pathway contributes to the generation of a Delta(pH) that induces thermal dissipation when Calvin cycle activity is reduced. Under these conditions, the PGR5 pathway also functions to limit the overreduction of the acceptor side of photosystem I, thus preventing photosystem I photoinhibition.
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        The dynamics of photosynthesis.

        Despite recent elucidation of the three-dimensional structure of major photosynthetic complexes, our understanding of light energy conversion in plant chloroplasts and microalgae under physiological conditions requires exploring the dynamics of photosynthesis. The photosynthetic apparatus is a flexible molecular machine that can acclimate to metabolic and light fluctuations in a matter of seconds and minutes. On a longer time scale, changes in environmental cues trigger acclimation responses that elicit intracellular signaling between the nucleo-cytosol and chloroplast resulting in modification of the biogenesis of the photosynthetic machinery. Here we attempt to integrate well-established knowledge on the functional flexibility of light-harvesting and electron transfer processes, which has greatly benefited from genetic approaches, with data derived from the wealth of recent transcriptomic and proteomic studies of acclimation responses in photosynthetic eukaroytes.
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          PROTON GRADIENT REGULATION5 is essential for proper acclimation of Arabidopsis photosystem I to naturally and artificially fluctuating light conditions.

          In nature, plants are challenged by constantly changing light conditions. To reveal the molecular mechanisms behind acclimation to sometimes drastic and frequent changes in light intensity, we grew Arabidopsis thaliana under fluctuating light conditions, in which the low light periods were repeatedly interrupted with high light peaks. Such conditions had only marginal effect on photosystem II but induced damage to photosystem I (PSI), the damage being most severe during the early developmental stages. We showed that PROTON GRADIENT REGULATION5 (PGR5)-dependent regulation of electron transfer and proton motive force is crucial for protection of PSI against photodamage, which occurred particularly during the high light phases of fluctuating light cycles. Contrary to PGR5, the NAD(P)H dehydrogenase complex, which mediates cyclic electron flow around PSI, did not contribute to acclimation of the photosynthetic apparatus, particularly PSI, to rapidly changing light intensities. Likewise, the Arabidopsis pgr5 mutant exhibited a significantly higher mortality rate compared with the wild type under outdoor field conditions. This shows not only that regulation of PSI under natural growth conditions is crucial but also the importance of PGR5 in PSI protection.
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            Author and article information

            Affiliations
            [1 ]ISNI 0000 0001 2172 9288, GRID grid.5949.1, Institute of Plant Biology and Biotechnology, , University of Münster, ; Schlossplatz 8, 48143 Münster, Germany
            [2 ]ISNI 0000 0004 0373 3971, GRID grid.136593.b, Institute for Protein Research, , Osaka University, ; 3-2 Yamadaoka, Suita-shi, Osaka, 565-0871 Japan
            [3 ]ISNI 0000 0004 0490 981X, GRID grid.5570.7, Department of Plant Biochemistry, , Ruhr-University Bochum, ; Universitätsstrasse 150, 44801 Bochum, Germany
            [4 ]ISNI 0000 0001 1033 6139, GRID grid.268441.d, Structural Epigenetics Laboratory, Graduate School of Medical Life Science, , Yokohama City University, ; 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045 Japan
            [5 ]ISNI 0000 0001 2171 1133, GRID grid.4868.2, School of Biological and Chemical Sciences, , Queen Mary University of London, ; Mile End Road, London, E1 4NS UK
            Contributors
            + 49 - 251 - 83 2 4790 , mhippler@uni-muenster.de
            Journal
            Photosynth Res
            Photosyn. Res
            Photosynthesis Research
            Springer Netherlands (Dordrecht )
            0166-8595
            1573-5079
            7 June 2017
            7 June 2017
            2017
            : 134
            : 3
            : 291-306
            28593495
            5683061
            408
            10.1007/s11120-017-0408-5
            © The Author(s) 2017

            Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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            © Springer Science+Business Media B.V., part of Springer Nature 2017

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