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      Species Variation in the Binding of hGH to Hepatic Membranes

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          The binding of <sup>125</sup>I-labeled human growth hormone (hGH) to liver membranes from several different species was studied to determine the lactogenic or somatotropic hormone nature of the receptors. Liver membranes from several species of the class of Mammalia bound significant quantities of <sup>125</sup>I-hGH. Goat, sheep, rat, mouse, and rabbit liver membranes exhibited the highest binding with cow, pig, human, and hamster liver membranes exhibiting severalfold less binding. The binding of the dog and cat liver membranes exhibited relatively high nonspecific binding. Fish and chicken liver membranes did not bind appreciable quantities of <sup>125</sup>I-hGH. In all species except for dog and cat in which <sup>125</sup>I-hGH bound to the membranes, hGH was the most effective competitor for binding. The mean ID<sub>50</sub> for hGH and all membranes was 2.4 × 10<sup>-9</sup> M. Human liver membranes exhibited the smallest ID<sub>50</sub>, 4.9 × 10<sup>-10</sup> M. In sheep liver membranes, bovine growth hormone (bGH) was equipotent to hGH in competing for <sup>125</sup>I-hGH binding. bGH also demonstrated significant competition for <sup>125</sup>I-hGH binding in pig and cow membranes. Ovine prolactin (oPrl) exhibited significant competition for <sup>125</sup>I-hGH only in rodent membranes. The ID<sub>50</sub> for oPrl was 3- to 10-fold greater than for hGH in the rat, hamster, and mouse liver membranes. The ID<sub>50</sub> for oPrl in the sheep liver membranes was 13-fold greater than that of hGH. We conclude the following: There appears to be a species specificity of hGH binding that may be phylogenetically significant and may result from variations in the structure of the hormone or the receptor. The competitive binding properties of hGH are fairly consistent within phylogenetic orders. (3) The simple designation of lactogenic or somatotropic for hormones and receptors is insufficient to characterize the binding properties of this group of hormones

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          Author and article information

          Horm Res Paediatr
          Hormone Research in Paediatrics
          S. Karger AG
          26 November 2008
          : 21
          : 1
          : 33-45
          Section of Pediatric Endocrinology and Ralph L. Smith Research Center, University of Kansas Medical Center, Kansas City, Kans., USA
          180022 Horm Res 1985;21:33–45
          © 1985 S. Karger AG, Basel

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          Pages: 13


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