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      Species Variation in the Binding of hGH to Hepatic Membranes

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          Abstract

          The binding of <sup>125</sup>I-labeled human growth hormone (hGH) to liver membranes from several different species was studied to determine the lactogenic or somatotropic hormone nature of the receptors. Liver membranes from several species of the class of Mammalia bound significant quantities of <sup>125</sup>I-hGH. Goat, sheep, rat, mouse, and rabbit liver membranes exhibited the highest binding with cow, pig, human, and hamster liver membranes exhibiting severalfold less binding. The binding of the dog and cat liver membranes exhibited relatively high nonspecific binding. Fish and chicken liver membranes did not bind appreciable quantities of <sup>125</sup>I-hGH. In all species except for dog and cat in which <sup>125</sup>I-hGH bound to the membranes, hGH was the most effective competitor for binding. The mean ID<sub>50</sub> for hGH and all membranes was 2.4 × 10<sup>-9</sup> M. Human liver membranes exhibited the smallest ID<sub>50</sub>, 4.9 × 10<sup>-10</sup> M. In sheep liver membranes, bovine growth hormone (bGH) was equipotent to hGH in competing for <sup>125</sup>I-hGH binding. bGH also demonstrated significant competition for <sup>125</sup>I-hGH binding in pig and cow membranes. Ovine prolactin (oPrl) exhibited significant competition for <sup>125</sup>I-hGH only in rodent membranes. The ID<sub>50</sub> for oPrl was 3- to 10-fold greater than for hGH in the rat, hamster, and mouse liver membranes. The ID<sub>50</sub> for oPrl in the sheep liver membranes was 13-fold greater than that of hGH. We conclude the following: There appears to be a species specificity of hGH binding that may be phylogenetically significant and may result from variations in the structure of the hormone or the receptor. The competitive binding properties of hGH are fairly consistent within phylogenetic orders. (3) The simple designation of lactogenic or somatotropic for hormones and receptors is insufficient to characterize the binding properties of this group of hormones

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1985
          1985
          26 November 2008
          : 21
          : 1
          : 33-45
          Affiliations
          Section of Pediatric Endocrinology and Ralph L. Smith Research Center, University of Kansas Medical Center, Kansas City, Kans., USA
          Article
          180022 Horm Res 1985;21:33–45
          10.1159/000180022
          3972335
          © 1985 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 13
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