5
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Sevoflurane induces endoplasmic reticulum stress mediated apoptosis inmouse hippocampal neuronal HT22 cells via modulating miR-15b-5p/Rab1A signaling pathway

      research-article

      Read this article at

      ScienceOpenPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Sevoflurane (Sev) is a widely used anaesthetic agent in clinical patients. Growing evidences indicated that Sev resulted in cognitive impairment via inducing endoplasmic reticulum (ER) stress mediated neurons apoptosis in vivo. However, the underlying molecular mechanisms have not yet fully understood. In this study, we found that Sev exposure suppresses cell viability, and induces apoptosis by activating caspase-3 apoptotic signaling pathway. Our results further verified that Sevtriggers ER stress via upregulating its markers glucose-regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), caspase-12 and cleaved-PARP proteins. Recently, microRNAs (miRNAs) have been proven to regulate ER stress in a variety of cells, especially neuronal cells. Therefore, we performed the microarray analysis to identified miRNA levels in HT22 cells after treatment with Sev. Our results showed that Sev induces miRNAs aberrant expression and miR-15b-5p was one of the miRNAs being most upregulated in HT22 cells. Furthermore, the Sev-induced apoptosis and ER stress were rescued by knockdown of miR-15b-5p. Additionally, we demonstrated that miR-15b-5p suppresses Rab1A, a regulator in inducing ER stress, by directly targeting its 3’-UTR in HT22 cells. These results suggested that Sev exposure induces ER stress mediated apoptosis in HT22 cells via regulating miR-15b-5p/Rab1A signaling pathway. These data may provide an important therapeutic strategy for fighting against Sev through ER stress mediated neuronal apoptosis in clinical patients.

          Related collections

          Author and article information

          Journal
          Int J Clin Exp Pathol
          Int J Clin Exp Pathol
          ijcep
          International Journal of Clinical and Experimental Pathology
          e-Century Publishing Corporation
          1936-2625
          2017
          01 August 2017
          : 10
          : 8
          : 8270-8280
          Affiliations
          Department of Anesthesiology, The People’s Hospital of Ruian (The Third Affiliated Hospital of Wenzhou Medical University) Ruian, Zhejiang, China
          Author notes
          Address correspondence to: Xuzhong Zhang, Department of Anesthesiology, The People’s Hospital of Ruian (The Third Affiliated Hospital of Wenzhou Medical University), NO. 108, Wangsong Road, Ruian 325200, Zhejiang, China. Tel: +86-577-65623293; E-mail: rahos@ 123456mail.wzptt.zj.cn
          [*]

          Equal contributors.

          Article
          PMC6965408 PMC6965408 6965408
          6965408
          31966678
          eed697f6-6ea5-4115-a77f-84a4df12ebc2
          IJCEP Copyright © 2017
          Categories
          Original Article

          endoplasmic reticulum stress,Sevoflurane,Rab1A,miR-15b-5p,neurons apoptosis

          Comments

          Comment on this article