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      Hydrogen peroxide inhibits epidermal growth factor receptor internalization in human fibroblasts.

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          Abstract

          Several cellular signal transduction cascades are affected by oxidative stress. In this study, the effect of hydrogen peroxide (H2O2) on the endocytosis of the epidermal growth factor (EGF) receptor was investigated. Exposure of HER14 cells to H2O2 resulted in a concentration-dependent inhibition of EGF receptor internalization. Binding studies demonstrated that this H2O2-induced inhibition in internalization was not due to altered binding of EGF to its receptor. Addition of H2O2 at different time points during internalization showed that EGF receptor internalization was rapidly reduced, suggesting that one of the first steps in the internalization process is inhibited. In addition, H2O2 inhibited the internalization of a different receptor, the chicken hepatic lectin receptor, in a concentration-dependent manner as well. Treatment of cells with another inducer of oxidative stress, cumene hydroperoxide, also resulted in a decreased internalization. Finally, we showed that H2O2 inhibited EGF-induced mono-ubiquitination of the EGF receptor pathway substrate clone 15, a process that normally occurs during EGF receptor endocytosis. These results clearly show that oxidative stress interferes with EGF signaling.

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          Author and article information

          Journal
          Free Radic. Biol. Med.
          Free radical biology & medicine
          0891-5849
          0891-5849
          Jan 01 2000
          : 28
          : 1
          Affiliations
          [1 ] Institute of Biomembranes, Department of Molecular Cell Biology, Utrecht University, The Netherlands. r.dewit@bio.uu.nl
          Article
          S0891-5849(99)00199-9
          10656288
          ef073aec-2389-45ec-be85-e10c39ea9f52
          History

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