A checklist of bats from Distrito Federal (DF) and Goiás state (GO) and, particularly a single cave (Passa Três cave), located in São Domingos karst area (GO), central Brazil, is presented. Data is based on literature and surveys carried out during 2,000 years. In total, 66 species were recorded, with 30 using caves as shelters. Passa Três cave harbors nine Phyllostomidae species; the most abundant species were Platyrrhinuslineatus, Lonchorhinaaurita, Desmodusrotundusand Carolliaperspicillata; and the less abundants Trachopscirrhosus, Anouracaudiferand Glossophagasoricina. Besides, the cave is shelter of two threatened bats - Lonchophylladekeyseri(Endangered category) and Lonchorhinaaurita(Vulnerable category), included at Brazilian List of Threatened Fauna, and of a rare species, Lionycterisspurrelli. Passa Três cave shows enough attributes to be considered as a SICOM (Sites of Importance for Conservation of Bats), which would ensure its protection.