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      Bio-Rad’s Bio-Plex® suspension array system, xMAP technology overview

      Archives of Physiology and Biochemistry

      Informa Healthcare

      Immunoassay, multiplex detection, ELISA, Bio-Plex®, xMAP, Luminex

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          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The Bio-Plex® system utilizes xMAP technology to permit the multiplexing of up to 100 different analytes. Multiplex analysis gives researchers the ability to look at analytes simultaneously providing more information from less sample volume in less time than traditional immunoassay methods. Similar to ELISA, xMAP utilizes an antibody sandwich for detection but differs from ELISA in capture substrate and detection method. Rather than a flat surface, Bio-Plex®assays make use of differentially detectable bead sets as a substrate capturing analytes in solution and employs fluorescent methods for detection. These bead sets identify the analytes and detection antibodies are used to measure the quantity of analyte. The use of differentially detectable beads enables the simultaneous identification and quantification of many analytes in the same sample.

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          Most cited references 3

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          Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.

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            Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA).

             M Lequin (2005)
            This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and Eva Engvall at Stockholm University in Sweden and by the research group of Anton Schuurs and Bauke van Weemen in The Netherlands. Today, fully automated instruments in medical laboratories around the world use the immunoassay principle with an enzyme as the reporter label for routine measurements of innumerable analytes in patient samples. The impact of EIA/ELISA is reflected in the overwhelmingly large number of times it has appeared as a keyword in the literature since the 1970s. Clinicians and their patients, medical laboratories, in vitro diagnostics manufacturers, and worldwide healthcare systems owe much to these four inventors.
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              Immunoassay using antigen-enzyme conjugates.

               K Dun,  T A Schuurs (1971)
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                Author and article information

                Journal
                Arch Physiol Biochem
                Arch. Physiol. Biochem
                napb
                Archives of Physiology and Biochemistry
                Informa Healthcare
                1381-3455
                1744-4160
                October 2012
                2 August 2012
                : 118
                : 4
                : 192-196
                Affiliations
                Bio-Rad Laboratories, Inc., Hercules, California, USA
                Author notes
                Address for Correspondence: Brett Houser, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547, USA 510–741–5613. E-mail: Brett_houser@ 123456Bio-Rad.com
                Article
                10.3109/13813455.2012.705301
                3469222
                22852821
                © 2012 Informa UK Ltd.

                This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Informa Healthcare journals , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                Categories
                Original Article

                Biochemistry

                immunoassay, xmap, luminex, elisa, multiplex detection, bio-plex®

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