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      Optimization of polysaccharides from Zagros oak leaf using RSM: Antioxidant and antimicrobial activities

      Carbohydrate Polymers

      Elsevier BV

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          Abstract

          Ultrasonic assisted-extraction technique was applied to extract the polysaccharide from Zagros oak (Quercus brantii Lindl). The effects of four independent factors (ultrasonic power (X1: 150-300 W), extraction temperature (X2: 50-90°C), extraction time (X3: 30-90 min), and the ratio of water to raw material (X4: 15-45)) on the extraction yield of polysaccharide from the leaves of Q. brantii Lindl (QBLP) were optimized using response surface methodology. The experimental data obtained were fitted to a second-order polynomial equation. The optimal extraction conditions for QBLP were determined as follows: X1: 205.8 W, X2: 81.9°C, X3: 55.6 min and X4: 23.4. Under these optimal conditions, the experimental yield was 19.42 ± 0.53%, which was well matched with the value predicted by the model 19.61%. The results indicated that polysaccharide has strong scavenging activities in vitro on DPPH and hydroxyl radicals. In addition, the QBLP showed good antimicrobial activity at 1.5-2.5mg/mL.

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          Author and article information

          Journal
          Carbohydrate Polymers
          Carbohydrate Polymers
          Elsevier BV
          01448617
          June 2014
          June 2014
          : 106
          : 238-246
          Article
          10.1016/j.carbpol.2014.02.028
          24721074
          © 2014

          https://www.elsevier.com/tdm/userlicense/1.0/

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