Tae Hyung Kim 1 , Eun-Ju Lee 2 , Ji-Hye Choi 2 , 3 , Sun Young Yim 1 , Sunwon Lee 4 , Jaewoo Kang 4 , Yoo Ra Lee 1 , Han Ah Lee 1 , Hyuk Soon Choi 1 , Eun Sun Kim 1 , Bora Keum 1 , Yeon Seok Seo 1 , Hyung Joon Yim 1 , Yoon Tae Jeen 1 , Hoon Jai Chun 1 , Hong Sik Lee 1 , Chang Duck Kim 1 , Hyun Goo Woo 2 , 3 , * , Soon Ho Um 1 , *
5 July 2018
The seroclearance of hepatitis B virus (HBV) surface antigen (HBsAg) is regarded as a functional cure of chronic hepatitis B (CHB) although it occurs rarely. Recently, several genome-wide association studies (GWASs) revealed various genetic alterations related to the clinical course of HBV infection. However, all of these studies focused on the progression of HBV infection to chronicity and had limited application because of the heterogeneity of HBV genotypes. In the present study, we aimed to determine susceptibility genetic markers for seroclearance of HBsAg in CHB patients with a homogenous viral genotype.
One hundred patients with CHB who had experienced HBsAg seroclearance before 60 years of age and another 100 with CHB showing high serum levels of HBsAg even after 60 years of age were enrolled. Extreme-phenotype GWAS was conducted using blood samples of participants.
We identified three single nucleotide polymorphisms, rs7944135 ( P = 4.17 × 10 −6, odds ratio [OR] = 4.16, 95% confidence interval [CI] = 2.27–7.63) at 11q12.1, rs171941 ( P = 3.52×10 −6, OR = 3.69, 95% CI = 2.13–6.42) at 5q14.1, and rs6462008 ( P = 3.40×10 −6, OR = 0.34, 95% CI = 0.22–0.54) at 7p15.2 as novel susceptibility loci associated with HBsAg seroclearance in patients with CHB. The flanking genes at these loci including MPEG1, DTX4, MTX3, and HOXA13 were suggested to have functional significance. In addition, through functional analysis, CXCL13 was also presumed to be related.