Genetic diversity and population structure of the endangered species Paeonia decomposita endemic to China and implications for its conservation

Abstract

Background

Paeonia decomposita, endemic to China, has important ornamental, medicinal, and economic value and is regarded as an endangered plant. The genetic diversity and population structure have seldom been described. A conservation management plan is not currently available.

Results

In the present study, 16 pairs of simple sequence repeat (SSR) primers were used to evaluate the genetic diversity and population structure. A total of 122 alleles were obtained with a mean of 7.625 alleles per locus. The expected heterozygosity ( H _e) varied from 0.043 to 0.901 (mean 0.492) in 16 primers. Moderate genetic diversity ( H _e = 0.405) among populations was revealed, with Danba identified as the center of genetic diversity. Mantel tests revealed a positive correlation between geographic and genetic distance among populations ( r = 0.592, P = 0.0001), demonstrating consistency with the isolation by distance model. Analysis of molecular variance (AMOVA) indicated that the principal molecular variance existed within populations (73.48%) rather than among populations (26.52%). Bayesian structure analysis and principal coordinate analysis (PCoA) supported the classification of the populations into three clusters.

Conclusions

This is the first study of the genetic diversity and population structure of P. decomposita using SSR. Three management units were proposed as conservation measures. The results will be beneficial for the conservation and exploitation of the species, providing a theoretical basis for further research of its evolution and phylogeography.