Catheter related infections (CRI) and bloodstream infection (BSI) associated to central venous catheter (CVC) is cause of frequent hospital-acquired infection (HAI) and a major reason of morbidity and mortality among patients and it is itself an indicator of quality health care. To determine the incidence of CRI and BSI and to identify the hospital areas where they occurred over a five-year period. A prospective study from 2004 to 2008 in a population of hospitalized patients who required the use of CVC was made. The HAI team detected the appearance of CRI during a daily routine surveillance. During the period of observation, 176 BSI were detected, accounting for 11 % of all HAI, and represented the third place in frequency. Incidence rate for 1000 catheter-days varied from 2.7 in 2006 to 5.4 in 2008; 57 % of these BSI were detected in the intensive care units. Candida spp. accounted as the more frequently isolated microorganism with predominance of C. parapsilosis. Our results and the incidence rate found in our study occurred within rates established in other studies.