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      Evaluation of new technique of sterilization using biological indicator

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          Abstract

          Background:

          A novel technique of sterilization of endodontic files is introduced in this article.

          Aims:

          Newly introduced sterilization unit, named “SteriFast” is compared with autoclave and glass bead sterilizer using biological indicator.

          Materials and Methods:

          Spore strips of Bacillus pumilus were cultured in nutrient broth. This cultured media was used to contaminate the experimental samples of endodontic files. These contaminated files were sterilized using three different techniques. The sterilized files were transferred into nutrient medium under aseptic condition. The results were observed after 24 h, 48 h, and 7 days.

          Results:

          The results showed that autoclave and new sterilization device (SteriFast) showed complete sterilization. The files sterilized using glass bead sterilizer showed bacterial growth (80%).

          Conclusions:

          Thus, it proves that autoclave and SteriFast are ideal techniques of sterilization of endodontic files. Glass bead sterilizer does not completely sterilize the files. The article also compares SteriFast and autoclave in other aspects such as its design, basic principle, advantages, and disadvantages. The article also describes features and design of SteriFast, used for all kind of small dental instruments.

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          Most cited references14

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          Microbiological evaluation of endodontic files after cleaning and steam sterilization procedures.

          Infection control procedures are essential for modern dental practice and they are continually evolving to meet the dental profession's high standards. The present study evaluated the efficacy of two cleaning procedures to reduce bacterial numbers on endodontic files, and evaluated the effect of biological debris on the subsequent sterilization of files.
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            • Article: not found

            Sterilizing Endodontic Files by four different sterilization methods to prevent cross-infection - An In-vitro Study.

            Aim of the study was to compare 4 different methods of sterilizing endodontic files in dental practice.
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              The sterilization of endodontic hand files.

              Several different methods of file sterilization were analyzed to determine the best method of providing complete file sterility, including the metal shaft and plastic handle. Six test groups of 15 files were studied using Bacillus stearothermophilus as the test organism. Groups were "sterilized" by glutaraldehyde immersion, steam autoclaving, and various techniques of salt sterilization. Only proper steam autoclaving reliably produced completely sterile instruments. Salt sterilization and glutaraldehyde solutions may not be adequate sterilization methods for endodontic hand files and should not be relied on to provide completely sterile instruments.
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                Author and article information

                Journal
                J Conserv Dent
                J Conserv Dent
                JCD
                Journal of Conservative Dentistry : JCD
                Medknow Publications & Media Pvt Ltd (India )
                0972-0707
                0974-5203
                Sep-Oct 2017
                : 20
                : 5
                : 346-350
                Affiliations
                [1]Department of Conservative Dentisty and Endodontics, VSPM Dental College and Research Center, Nagpur, Maharashtra, India
                Author notes
                Address for correspondence: Dr. Nomal Chintan Sheth, B2 Madhu Kunj, Navroji Cross Lane, Ghatkopar (w), Mumbai - 400 086, Maharashtra, India. E-mail: drnomalshah@ 123456gmail.com
                Article
                JCD-20-346
                10.4103/JCD.JCD_253_16
                5767831
                29386784
                efb96d40-011e-491e-8bd0-17c0196cfc58
                Copyright: © 2017 Journal of Conservative Dentistry

                This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

                History
                : 09 November 2016
                : 29 October 2017
                : 27 November 2017
                Categories
                Original Research Article

                Dentistry
                autoclave,bacillus pumilus,biological indicator,sterifast,sterilization,ultraviolet germicidal irradiation

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