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      Identification and Functional Characterization of a Novel Δ12 Fatty Acid Desaturase Gene from Haematococcus pluvialis

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          Abstract

          The freshwater microalga Haematococcus pluvialis accumulates large amounts of fatty acids in response to adverse conditions. However, the key fatty acid desaturase genes in H. pluvialis remain unknown. In this study, we cloned and functionally characterized a Δ12 fatty acid desaturase gene, and designated it as HpFAD2. The open reading frame of HpFAD2 consisted of 1137 base pairs and encoded 378 amino acids. The deduced polypeptide showed 70% identity to other endoplasmic reticulum Δ12 fatty acid desaturases, whereas it had only 44% identity to plastid Δ12 fatty acid desaturases. The PSORT algorithm and phylogenetic analysis further confirmed its affiliation to the endoplasmic reticulum Δ12 fatty acid desaturases. Heterologous expression was performed in Saccharomyces cerevisiae cells transformed with the recombinant plasmid pYES2-HpFAD2. Two additional fatty acids (C16:2 and C18:2) were detected in the yeast transformants. The results indicated Δ12 desaturation activity and substrate preference for C18:1 over C16:1. The transcriptional levels of H. pluvialis HpFAD2 at different growth stages were measured by quantitative polymerase chain reaction (PCR), indicating that the HpFAD2 transcriptional levels were significantly higher in red cells than those in green cells. Our study brings more insight into the fatty acid biosynthetic pathway of H. pluvialis.

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          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          15 November 2020
          01 December 2020
          : 19
          : 6
          : 1362-1370
          Affiliations
          1School of Marine Sciences, Ningbo University, Ningbo 315211, China
          2College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China
          Author notes
          *Corresponding author: XU Jilin, Tel: 0086-574-87609570, E-mail: xujilinnbu@ 123456163.com
          Article
          s11802-020-4418-0
          10.1007/s11802-020-4418-0
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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          Self URI (journal-page): https://www.springer.com/journal/11802

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