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      Communication of Agonist-Induced Electrical Responses along ‘Capillaries’ in vitro Can Be Modulated by Lipopolysaccharide, but Not Nitric Oxide


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          Communication of agonist-induced membrane potential changes along blood vessels has been proposed to contribute to the coordination of microvascular function. Factors mediating septic shock may compromise this coordination. Using electrophysiology in a simplified in vitro model of endothelial cells grown as capillary-like structures, we aimed to determine (i) the effect of lipopolysaccharide (LPS) on endothelial cell membrane potential responses to ATP and KCl and (ii) the effect of LPS and nitric oxide (NO) on cell-to-cell communication. Treatment of ‘capillaries’ with LPS (10 µg/ml for 1 h) did not affect local responsiveness to ATP or KCl, but reduced cell communication by a tyrosine-kinase-dependent mechanism. Treatment of ‘capillaries’ with the NO donor DETA (100 µ M) or the NO synthase inhibitor L-NAME (100 µ M) had no effect on cell communication or the response to LPS. Endogenous NO production, stimulated by LPS + interferon-γ (100 U/ml) treatment, also had no effect on cell communication beyond that of LPS alone. We conclude that LPS, but not NO, can modulate conduction of agonist-induced electrical responses along endothelial capillary-like structures in vitro.

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          Author and article information

          J Vasc Res
          Journal of Vascular Research
          S. Karger AG
          October 2002
          18 September 2002
          : 39
          : 5
          : 405-413
          aChild Health Research Institute, bLawson Health Research Institute and Departments of cMedical Biophysics, dPediatrics and ePhysiology, University of Western Ontario, London, Ont., Canada
          64519 J Vasc Res 2002;39:405–413
          © 2002 S. Karger AG, Basel

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          Page count
          Figures: 7, References: 37, Pages: 9
          Research Paper


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