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      Facile synthesis of β-lactoglobulin-functionalized multi-wall carbon nanotubes and gold nanoparticles on glassy carbon electrode for electrochemical sensing.

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          Abstract

          A facile approach was developed for the preparation of nanocomposite based on β-lactoglobulin (BLG)-functionalized multi-wall carbon nanotubes (MWCNTs) and gold nanoparticles (GNPs) for the first time. Owing to the amphipathic nature, BLG can be adopted onto the surface of MWCNTs to form BLG-MWCNTs with uniform dispersion in water. Taking advantage of sulfhydryl groups on BLG-MWCNTs, GNPs were decorated on the BLG-MWCNTs-modified glassy carbon electrode (GCE) by electrodeposition. The nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy and X-ray spectroscopy analysis. Cyclic voltammetry and chronoamperometric method were used to evaluate the electrocatalytic ability of the nanocomposite. Furthermore, a glucose biosensor was developed based on the immobilization of glucose oxidase with cross-linking in the matrix of bovine serum albumin (BSA) on the nanocomposite modified GCE. The resulting biosensor exhibited high sensitivity (3.98 μA mM(-1)), wider linear range (0.025-5.5 mM), low detection limit (1.1 μM at the signal-to-noise ratio of 3) and fast response time (within 7s) for glucose detection.

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          Author and article information

          Journal
          Biosens Bioelectron
          Biosensors & bioelectronics
          1873-4235
          0956-5663
          Dec 15 2014
          : 62
          Affiliations
          [1 ] The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, PR China.
          [2 ] College of Acumox and Tuina, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, PR China.
          [3 ] The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, PR China. Electronic address: qiangchen@nankai.edu.cn.
          Article
          S0956-5663(14)00443-6
          10.1016/j.bios.2014.06.030
          24984286
          f07a8dc3-b777-45ed-b7cd-2308e8c63510
          Copyright © 2014 Elsevier B.V. All rights reserved.
          History

          Biosensor,Gold nanoparticles,Multi-wall carbon nanotube,Nanocomposite,β-Lactoglobulin

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