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      Constant, but Not Pulsed Calcitriol Suppresses Hemodialysis Patients’ Antigen-Induced Lymphocyte Proliferation

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          Abstract

          Background/Aims: In vitro constant calcitriol [1,25-(OH)<sub>2</sub>D<sub>3</sub>] inhibits healthy individuals’ T lymphocyte proliferation at supraphysiological concentrations. In contrast, among hemodialysis patients, intravenous 1,25-(OH)<sub>2</sub>D<sub>3</sub> pulse therapy of secondary hyperparathyroidism has been shown to be even immunostimulatory. We studied the effect of in vitro constant and intermittent 1,25-(OH)<sub>2</sub>D<sub>3</sub> on lymphocyte antigen response of hemodialysis patients. Methods: Twelve hemodialysis patients’ peripheral blood mononuclear cells were stimulated with purified protein derivative of tuberculin (12.5, 25 and 50 mg/l) or tetanus toxoid (TT; 1,000, 5,000 and 10,000 Lf/l, limit of flocculation) for 7 days. Constant 1,25-(OH)<sub>2</sub>D<sub>3</sub> was added to all cultures at concentrations of 0, 10<sup>–10</sup> or 0.25 × 10<sup>–9</sup> mol/l (0, 42 and 105 ng/l) and to half of the cultures additionally as a 0.75 × 10<sup>–9</sup> mmol/l (315-ng/l) pulse on the 5th culture day. Results: TT-induced lymphocyte proliferation was statistically related to a constant 1,25-(OH)<sub>2</sub>D<sub>3</sub> concentration (p = 0.001, analysis of variance). With constant 1,25-(OH)<sub>2</sub>D<sub>3</sub> concentrations of 0, 42 and 105 ng/l, the TT-induced responses were 1.53, 1.44 and 1.40 log cpm, respectively (mean of TT concentrations). The responses of the (additionally) pulse-treated cells [1.65, 1.50 and 1.40 log cpm; concentrations of constant 1,25-(OH)<sub>2</sub>D<sub>3</sub> as above] were similar to those of the nonpulsed cells. Thus constant, but not pulsed 1,25-(OH)<sub>2</sub>D<sub>3</sub> decreased the TT responses. On the purified protein derivative of tuberculin response, neither constant nor pulsed 1,25-(OH)<sub>2</sub>D<sub>3</sub> had any significant effect. Conclusions: The decline of TT response with constant 1,25-(OH)<sub>2</sub>D<sub>3</sub> corresponds with findings on immunosuppressive action of 1,25-(OH)<sub>2</sub>D<sub>3</sub> in previous studies done on normal subjects’ cells. This was not seen with intermittently applied 1,25-(OH)<sub>2</sub>D<sub>3</sub>. These results support the previous concept that intermittent 1,25(OH)<sub>2</sub>D<sub>3</sub> therapy is not immunosuppressive in hemodialysis patients.

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          Most cited references 3

          • Record: found
          • Abstract: not found
          • Article: not found

          The immunobiology ofvitamin D

           R.W. Baldwin (1988)
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            • Record: found
            • Abstract: found
            • Article: not found

            Immunological properties of vitamin D analogues and metabolites.

             Lise Binderup (1992)
            This commentary has attempted to describe some of the new aspects of our knowledge of the immunological properties of 1 alpha,25(OH)2D3, the physiologically active metabolite of vitamin D3, and its new analogues. These analogues will, in the future, serve as tools to increase our understanding of the role of vitamin D in immunobiology, not only in basal research but also, hopefully, in the therapy of immune-mediated diseases.
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              • Record: found
              • Abstract: not found
              • Article: not found

              Vitamin D and the immune system

               Edward Amento (1987)
                Bookmark

                Author and article information

                Journal
                NEF
                Nephron
                10.1159/issn.1660-8151
                Nephron
                S. Karger AG
                1660-8151
                2235-3186
                2000
                October 2000
                22 September 2000
                : 86
                : 2
                : 139-144
                Affiliations
                aDepartment of Medicine, Tampere University Hospital, Tampere, bTampere School of Public Health, and cInstitute of Medical Technology, University of Tampere, Finland
                Article
                45732 Nephron 2000;86:139–144@5L}@4E}
                10.1159/000045732
                11014983
                © 2000 S. Karger AG, Basel

                Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

                Page count
                Figures: 2, Tables: 3, References: 36, Pages: 6
                Product
                Self URI (application/pdf): https://www.karger.com/Article/Pdf/45732
                Categories
                Original Paper

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