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Abstract
An amperometric chloramphenicol (CAP) immunosensor was fabricated by covalently immobilizing
anti-chloramphenicol acetyl transferase (anti-CAT) antibody on cadmium sulfide nanoparticles
(CdS) modified-dendrimer that was bonded to the conducting polymer (poly 5, 2': 5',
2''-terthiophene-3'-carboxyl acid (poly-TTCA)) layer. The AuNPs, dendrimers, and CdS
nanoparticles were deposited onto the polymer layer in order to enhance the sensitivity
of the sensor probes. The particle sizes were determined using scanning electron microscopy
(SEM) and transmission electron microscopy (TEM). The immobilization of dendrimers,
CdS, and anti-CAT were confirmed using energy disruptive spectroscopy (EDS), X-ray
photoelectron spectroscopy (XPS), and quartz crystal microbalance (QCM) techniques.
The detection of CAP was based on the competitive immuno-interaction between the free-
and labeled-CAP for active sites of the anti-CAT. Hydrazine was used as the label
for CAP, and it electrochemically catalyzed the reduction of H(2)O(2) at -0.35 V vs.
Ag/AgCl. Under optimized conditions, the proposed immunosensor exhibited a linear
range of CAP detection between 50 pg/mL and 950 pg/mL, and the detection limit was
45 pg/mL. The immunosensor was examined in real meat samples for the analysis of CAP.
(c) 2009 Elsevier B.V. All rights reserved.