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      Nature of the Calcium Regulatory System of Bovine Arterial Actomyosin


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          A calcium-sensitive actomyosin was prepared from bovine aortic muscularis. The results of applying filament displacement tests indicate that bovine arterial actomyosin possesses predominantly a calcium regulatory system associated with the myosin filament, but also may possess a calcium regulatory system associated with the actin filament. Calcium binding to highly purified myosin preparations could not account for the calcium binding to calcium-sensitive actomyosin. A calcium-insensitive actomyosin preparation, prepared by omitting dithiothreitol from the isolation procedure, showed a definite loss of tropomyosin but no change in calcium binding relative to the calcium-sensitive actomyosin.

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          Author and article information

          J Vasc Res
          Journal of Vascular Research
          S. Karger AG
          19 September 2008
          : 16
          : 1
          : 26-34
          Department of Physiology, Medical College of Virginia, Richmond, Va.
          158187 Blood Vessels 1979;16:26–34
          © 1979 S. Karger AG, Basel

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          Pages: 9
          Original Paper


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