5
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Neurobrucellosis in a common bottlenose dolphin ( Tursiops truncatus) stranded in the Canary Islands

      case-report

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Background

          Brucella spp. isolation is increasingly reported in cetaceans, although associated pathologies, including lesions of the musculoskeletal and nervous systems, are less frequently described. Concerning the nervous system, Brucella sp. infection causing meningitis, meningoencephalitis or meningoencephalomyelitis have been extensively reported in striped dolphins ( Stenella coeruleoalba), and less frequently in other cetacean species.

          Case presentation

          A juvenile female common bottlenose dolphin ( Tursiops truncatus) was found stranded alive in Lanzarote (Canary Islands, Spain) in 2005, but died shortly after. On physical examination, the dolphin showed a moderate body condition and was classified as code 2 (fresh dead) at the time of necropsy. The main gross findings were severe multiorgan parasitism, thickened and congested leptomeninges, and (sero)fibrino-suppurative and proliferative arthritis of the shoulder joint. Histopathological examination revealed the distinct features of a sub-acute systemic disease associated with Cetacean Morbillivirus (CeMV) infection. However, brain lesions diverged from those reported in systemic CeMV infection. This led to suspect that there was a coinfecting pathogen, based on the characteristics of the inflammatory response and the lesion distribution pattern in the central nervous system. Brucella sp. was detected in the brain tissue by PCR and Brucella antigen was demonstrated by immunohistochemistry in the brain and shoulder joint lesions.

          Conclusions

          The zoonotic potential of marine mammal strains of Brucella has been demonstrated both in natural and laboratory conditions. In this study, PCR detected Brucella sp. in the brain of a common bottlenose dolphin stranded in the Canary Islands; the dolphin was also co-infected with CeMV. This is the first detection of Brucella sp. infection in a stranded cetacean in this archipelago. Therefore, we stress the importance of taking adequate measures during the handling of these species to prevent the transmissions of the infection to humans.

          Related collections

          Most cited references62

          • Record: found
          • Abstract: found
          • Article: not found

          Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts.

          Small Gram-negative cocco-bacilli resembling Brucella strains have been reported from marine mammals since the mid-1990s. Their placement in the genus Brucella has been supported by the following characteristics: they are aerobic, non-motile and catalase-positive, do not produce acid from carbohydrates and have a DNA-DNA relatedness value of >77% with the six established members of the genus. Twenty-eight European isolates of the genus Brucella from marine mammals were distinguished from the six recognized species by their pattern of utilization of eleven substrates in oxidative metabolism tests and phage lysis. The 28 strains could be further separated into two groups with cetaceans and seals as their respective preferred hosts on the basis of molecular methods and on differences in the metabolism of l-arabinose, d-galactose and d-xylose. The names Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. are proposed for the isolates from cetaceans and seals, respectively. The type strain of Brucella ceti sp. nov. is NCTC 12891T (=BCCN 94-74T) and the type strain of Brucella pinnipedialis sp. nov. is NCTC 12890T (=BCCN 94-73T).
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens.

            Brucella species are responsible for brucellosis, one of the world's most widespread zoonotic diseases causing abortion in domestic animals and a potentially debilitating infection of man. Despite the identification of a number of distinct species within the genus with differing host preferences and pathogenicity it has been known for many years that members of the genus are genetically homogeneous. However, since the start of the millennium the application of new technologies to the group has resulted in rapid advances in the understanding of Brucella diversity and, after many years of inactivity, a process of expansion of the genus is underway. This review summarises the current state of knowledge in this area and outlines how this informs understanding of the taxonomy and evolution of the group and is enabling the development of increasingly sophisticated molecular typing tools.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: found
              Is Open Access

              Human Neurobrucellosis with Intracerebral Granuloma Caused by a Marine Mammal Brucella spp.

              We present the first report of community-acquired human infections with marine mammal–associated Brucella spp. and describe the identification of these strains in two patients with neurobrucellosis and intracerebral granulomas. The identification of these isolates as marine mammal strains was based on omp2a sequence and amplification of the region flanking bp26.
                Bookmark

                Author and article information

                Contributors
                eva.sierra@ulpgc.es
                +34 928459712 , antonio.fernandez@ulpgc.es
                idaira.felipe101@alu.ulpgc.es
                daniele.zucca101@alu.ulpgc.es
                g.difrancesco@izs.it
                josue.diaz101@alu.ulpgc.es
                simona.sacchini@ulpgc.es
                miguel.rivero@ulpgc.es
                manuel.arbelo@ulpgc.es
                Journal
                BMC Vet Res
                BMC Vet. Res
                BMC Veterinary Research
                BioMed Central (London )
                1746-6148
                21 October 2019
                21 October 2019
                2019
                : 15
                : 353
                Affiliations
                [1 ]ISNI 0000 0004 1769 9380, GRID grid.4521.2, Division of Histology and Animal Pathology, , Institute for Animal Health and Food Security (IUSA), Veterinary School, Universidad de Las Palmas de Gran Canaria, ; 35416 Arucas, Gran Canaria, Canary Islands Spain
                [2 ]National and international Reference Laboratory for Brucellosis, Istituto Zooprofilattico Sperimentale Abruzzo e Molise, Teramo, Italy
                [3 ]ISNI 0000 0004 1937 0722, GRID grid.11899.38, Laboratory of Wildlife Comparative Pathology, , School of Veterinary Medicine and Animal Science, University of São Paulo, ; São Paulo, SP Brazil
                [4 ]ISNI 0000 0004 4687 2082, GRID grid.264756.4, Texas A&M Veterinary Medical Diagnostic Laboratory (TVMDL), ; College Station, TX USA
                Author information
                http://orcid.org/0000-0001-5281-0521
                Article
                2089
                10.1186/s12917-019-2089-0
                6805616
                31638986
                f125b0f4-b73d-494d-a272-0bf28d96ebfe
                © The Author(s). 2019

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

                History
                : 2 October 2018
                : 12 September 2019
                Funding
                Funded by: FundRef http://dx.doi.org/10.13039/501100010198, Ministerio de Economía, Industria y Competitividad, Gobierno de España;
                Award ID: CGL2015/71498P
                Award Recipient :
                Categories
                Case Report
                Custom metadata
                © The Author(s) 2019

                Veterinary medicine
                brucella,bottlenose dolphin,canary islands,cetacean morbillivirus,neurobrucellosis

                Comments

                Comment on this article