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      Ribosome profiling shows that miR-430 reduces translation before causing mRNA decay in zebrafish.

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          Abstract

          MicroRNAs regulate gene expression through deadenylation, repression, and messenger RNA (mRNA) decay. However, the contribution of each mechanism in non-steady-state situations remains unclear. We monitored the impact of miR-430 on ribosome occupancy of endogenous mRNAs in wild-type and dicer mutant zebrafish embryos and found that miR-430 reduces the number of ribosomes on target mRNAs before causing mRNA decay. Translational repression occurs before complete deadenylation, and disrupting deadenylation with use of an internal polyadenylate tail did not block target repression. Lastly, we observed that ribosome density along the length of the message remains constant, suggesting that translational repression occurs by reducing the rate of initiation rather than affecting elongation or causing ribosomal drop-off. These results show that miR-430 regulates translation initiation before inducing mRNA decay during zebrafish development.

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          Author and article information

          Journal
          Science
          Science (New York, N.Y.)
          American Association for the Advancement of Science (AAAS)
          1095-9203
          0036-8075
          Apr 13 2012
          : 336
          : 6078
          Affiliations
          [1 ] Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
          Article
          science.1215704 NIHMS429317
          10.1126/science.1215704
          3547538
          22422859
          f14af701-a556-4eea-9731-bbb0386f3e16
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