To compare the apical seal of lateral condensation technique, thermafil and one-step by using this model. A two-chamber bacterial microleakage model using E. faecalis as microbial marker was used for evaluation of the leakage. Bacterial penetration was monitored over a 60-day period. Leakage was recorded when turbidity was observed in the lower chamber. After comparing the bacterial penetration values, total penetration was observed 45% in lateral condensation technique, 80% in thermafil and 75% in one-step. There was no significant difference between groups after 60 days; however, the microleakage in lateral condensation group was seen later than one-step and thermafil. Thermafil and one-step obturator can be advocated as effective obturation techniques for achieving predictable success in endodontic therapy. Thermafil and one-step obturator are suitable devices for obturation.