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      Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain.

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          Abstract

          A novel biomimetic microsensor for measuring nitric oxide (NO) in the brain in vivo was developed. The sensor consists of hemin and functionalized multi-wall carbon nanotubes covalently attached to chitosan via the carbodiimide crosslinker EDC followed by chitosan electrodeposition on the surface of carbon fiber microelectrodes. Cyclic voltammetry supported direct electron transfer from the Fe(III)/Fe(II) couple of hemin to the carbon surface at -0.370 V and -0.305 V vs. Ag/AgCl for cathodic and anodic peaks, respectively. Square wave voltammetry revealed a NO reduction peak at -0.762 V vs. Ag/AgCl that increased linearly with NO concentration between 0.25 and 1 μM. The average sensitivity of the microsensors was 1.72 nA/μM and the limit of detection was 25 nM. Oxygen and hydrogen peroxide reduction peaks were observed at -0.269 V and -0.332 V vs. Ag/AgCl, respectively and no response was observed for other relevant interferents, namely ascorbate, nitrite and dopamine. The microsensor was successfully applied to the measurement of exogenously applied NO in the rat brain in vivo.

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          Author and article information

          Journal
          Biosens Bioelectron
          Biosensors & bioelectronics
          1873-4235
          0956-5663
          Jun 15 2013
          : 44
          Affiliations
          [1 ] Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
          Article
          S0956-5663(13)00017-1
          10.1016/j.bios.2013.01.015
          23419387
          f153e63e-2861-461b-9ff1-754ea5ec1bda
          Copyright © 2013 Elsevier B.V. All rights reserved.
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