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      Proopiomelanocortin Messenger RNA Levels Are Increased in the Anterior Pituitary of the Sheep Fetus after Adrenalectomy in Late Gestation

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          Abstract

          We have investigated the effect of bilateral adrenalectomy at 116–119 days’ gestation on the levels of the messenger (m) RNA for proopiomelanocortin (POMC) in the anterior pituitary of the fetal sheep and in the ovine placentome during late gestation (134–136 days’ gestation). After fetal adrenalectomy there was a significant (p < 0.001) and sustained increase in circulating ACTH concentrations in the adrenalectomised group (1,838 ± 155 ng/l at 130–136 days) when compared with the intact control group (131 ± 25 ng/l at 130–136 days). The mean levels of POMCmRNA relative to 18S RNA were also significantly higher (p < 0.001) in the adrenalectomised fetal sheep pituitaries (2.8 ± 0.12; n = 4) than in the intact/control fetal sheep pituitaries (1.31 ± 0.13; n = 4). In contrast to the findings in the anterior pituitary, POMCmRNA was not detected in RNA extracted from the placentomes of either the adrenalectomised or intact fetal sheep. There was also a significant arteriovenous difference in ACTH concentrations in the umbilical circulation in both adrenalectomised and intact fetal sheep at 134–136 days’ gestation. This study demonstrates therefore that the fetal adrenals act to suppress POMCmRNA levels in late gestation and also that the increase in circulating ACTH after adrenalectomy originates from the pituitary and not the placentome.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1990
          1990
          03 April 2008
          : 52
          : 3
          : 297-302
          Affiliations
          aDepartment of Physiology, Monash University, Clayton; bDepartment of Anatomy, University of Melbourne, Parkville; cMedical Research Centre, Prince Henry’s Hospital, Melbourne, Australia; dPhysiological Laboratory, Cambridge, UK
          Article
          125601 Neuroendocrinology 1990;52:297–302
          10.1159/000125601
          2170855
          © 1990 S. Karger AG, Basel

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          Page count
          Pages: 6
          Categories
          Original Paper

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