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      Flow Cytometric Analysis of Surface Antigens on Human Conjunctival Epithelial Cells

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          We analyzed the levels of expression of different surface adhesion molecules on normal human conjunctival epithelial cells with cytology and flow cytometry. The levels of β<sub>1</sub> (CD29), VLA-1 (CD49a), VLA-2 (CD49b), and VLA-3 (CD49c) integrins were high, whereas those of VLA-5 (CD49e) and VLA-6 (CD49f) were faint, and that of VLA-4 (CD49d) was undetectable. Among the β<sub>2</sub> integrins, the levels of Mac-1 (CD1 lb) and p 150.25 (CD 11 c) were positive, while LFA-1 (CD 11 a) was not detectable. HLA class I and class II were also expressed. However, CR2 (CD21), CD57, CD44 and ICAM-1 (CD54) were not detected. Our study suggests that these techniques may aid in identifying changes in the expression levels of various surface antigens on conjunctival epithelial cells that could occur in ocular surface disease.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          11 December 2009
          : 29
          : 2
          : 103-109
          aDepartment of Ophthalmology, Tokyo Dental College, Chiba, bSanten Pharmaceutical Co., Ltd., Nara Research and Development Center, Nara, and cDepartment of Internal Medicine, Saitama Medical Center, Saitama Medical School, Saitama, Japan
          268003 Ophthalmic Res 1997;29:103–109
          © 1997 S. Karger AG, Basel

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          Pages: 7
          Original Paper


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