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      An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosides.

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          Abstract

          We developed an atomic force microscopy (AFM) method to determine the binding forces between a model cell wall plasma membrane and Vibrio cholerae. V. cholerae cells were covalently attached to AFM probes and forces were determined against a lipid bilayer containing sialic acid (N-acetylneuraminic acid) molecules as well as several control surfaces.

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          Author and article information

          Journal
          J. Microbiol. Methods
          Journal of microbiological methods
          1872-8359
          0167-7012
          Feb 2011
          : 84
          : 2
          Affiliations
          [1 ] Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA. liz@dbi.udel.edu
          Article
          S0167-7012(10)00445-8
          10.1016/j.mimet.2010.12.014
          21192989
          Copyright © 2011 Elsevier B.V. All rights reserved.

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