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      Thymic stromal lymphopoietin contribution to the recruitment of circulating fibrocytes to the lung in a mouse model of chronic allergic asthma.

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          Abstract

          Objective: Fibrocyte localization to the airways and thymic stromal lymphopoietin (TSLP) overexpression in the lung are features of severe asthma. The aim of this study was to determine whether TSLP contributes to fibrocyte trafficking and airway remodeling in a mouse model of allergic asthma. Methods: We established a chronic asthma animal model by administering house dust mite (HDM) extracts intranasally for up to 5 consecutive weeks. Mouse anti-TSLP monoclonal antibody (mAb) was given intraperitoneally starting the 4th week. Fluorescence-labeled CD34/collagen I (Col I)-dual-positive fibrocytes were examined by confocal microscopy. The level of TGF-β1 in the bronchoalveolar lavage (BAL) fluid was determined by ELISA. Results: We found significantly increased levels of TSLP and TGF-β1 in the lung of the mice subjected to repeated allergen exposure, which was accompanied by increased number of fibrocytes in the sub-epithelial zone and the BAL fluid. However, blocking TSLP markedly decreased the production of TGF-β1, reduced the number of fibrocytes and subsequently prevented alterations of both airway and vascular structures. Conclusions: Our data suggested that TSLP might function in airway remodeling by promoting circulating fibrocyte recruitment to the lung in the mice subjected to chronic allergen exposure. These results provide a better rationale for targeting the interaction between TSLP and fibrocytes as a therapeutic approach for chronic allergic asthma.

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          Author and article information

          Journal
          J Asthma
          The Journal of asthma : official journal of the Association for the Care of Asthma
          Informa UK Limited
          1532-4303
          0277-0903
          September 2018
          : 55
          : 9
          Affiliations
          [1 ] a Department of Pulmonary Diseases, The Third Affiliated Hospital of Sun Yat-Sen University , Institute of Respiratory Diseases of Sun Yat-Sen University , Guangzhou , China.
          [2 ] b Department of Pediatrics , The Third Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.
          [3 ] c Department of Pulmonary Diseases, The First Affiliated Hospital of Sun Yat-Sen University , Institute of Respiratory Diseases of Sun Yat-Sen University , Guangzhou , China.
          [4 ] d Department of Otolaryngology, Yong Loo Lin School of Medicine , National University of Singapore , Singapore.
          Article
          10.1080/02770903.2017.1386213
          28972433
          f23ab8e2-ba7f-4b2c-b5f0-27cba0752b15
          History

          Airway remodeling,asthma,fibrocyte,house dust mite,thymic stromal lymphopoietin

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