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      Micropropagação de bananeira "prata" através de cultivo in vitro de ápices vegetativos Translated title: Micropropagation of banana prata through in vitro culture of vegetative apices

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          Abstract

          Ápices vegetativos de mudas de 2 a 4 meses de idade foram cultivados no meio MS com 30 g/l de sacarose e 22 µM benzilammopurina. Após 8 a 10 semanas, foram produzidos uma ou duas gemas laterais; a subcultura destas no mesmo meio produziu até 10 gemas após 6 a 10 semanas. A maior taxa de multiplicação em subculturas successivas foi de 40 plántulas novas por explante; a taxa de multiplicação média foi de 10 gemas novas por explante em 8 semanas. O enraizamento ocorreu no mesmo meio mas um melhor enraizamento foi observado nos meios diluidos pela metade da concentração de MS em presença de 1 µM AIB. As plántulas de diferentes subculturas transplantadas no campo apresentaram um crescimento mais uniforme e mais vigoroso do que as mudas tradicionais da mesma variedade. O sistema mostrou ser viável em escala comercial.

          Translated abstract

          Vegetative shoot tips from 2 to 4 month old suckers of banana cv. "Prata" were grown on MS medium with 30 g/l sucrose and 22 µM benzylaminopurine. After 8 to 10 weeks, one or two lateral buds were produced; subculture of these on the same medium produced up to 10 buds after 6 to 10 weeks. The highest multiplication factor observed in subsequent subcultures was 40 new palntlets per explant; the average multiplication factor was 10 buds per explant in 8 weeks. Rooting occurred on the same medium but better rooting was achieved on medium diluted to halfstrength MS with 1 µM indole-butyric acid. Plants obtained from different generations of subculture, when transplanted to the field, showed more uniform and more vigorous growth than traditional planting material of the same variety. The system is viable for commercial scale multiplication.

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          Most cited references14

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          A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (Banana and plantain)

          A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions is presented in this paper. Shoot-tip cultures of Musa cultivars (both banana and plantain) are induced by culturing small excised shoot apices on modified MS semisolid medium supplemented with various concentrations and combinations of auxins and cytokinins. The effects of cytokinin concentration in the medium as well as the genotypic configuration of the cultivars on the rate of shoot-bud proliferation have been tested. The established shoot-tip cultures grown on modified MS semisolid medium supplemented with IAA (0.18 mg/l) and BA (2.30 mg/l) have been successfully stored at 15°C with 1000 lux light intensity up to 13-17 months depending on the cultivar. The cultivars tested in the present investigation seem to vary in their ability to withstand minimal growth temperature.
            • Record: found
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            In vitro propagation of banana (Musa spp.): initiation, proliferation and development of shoot-tip cultures on defined media

            W.C. Wong (1986)
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              • Abstract: not found
              • Article: not found

              Tissue-culture propagation of banana

                Author and article information

                Journal
                abb
                Acta Botanica Brasilica
                Acta Bot. Bras.
                Sociedade Botânica do Brasil (Alta Floresta, MT, Brazil )
                0102-3306
                1677-941X
                July 1989
                : 3
                : 1
                : 121-130
                Affiliations
                [01] Brasília DF orgnameUniversidade de Brasília orgdiv1Depto. de Biologia Vegetal
                [02] Campinas SP orgnameBioplanta Tecnologia de Plantas Ltda
                Article
                S0102-33061989000100006 S0102-3306(89)00300106
                f278effe-c131-414d-b600-a3566223cc99

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 20 July 1988
                : 01 June 1989
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 15, Pages: 10
                Product

                SciELO Brazil


                tissue culture,banana cv. "Prata",Musa spp,multiplicação clonal,cultura de tecidos,bananeira "prata",micropropagation

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