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      Identification of birch pollen species using FTIR spectroscopy

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          In this study, the morphology and chemical composition of pollen grains of six birch species ( Betula utilis Doorenbos, B. dahurica, B. maximowicziana, B. pendula, B. pubescens and B. humilis) were examined to verify which of these features allow distinguishing them in a more unambiguous way. For this purpose, scanning electron microscopy and light microscopy, as well as Fourier transform infrared (FTIR) spectroscopy and curve-fitting analysis of amide I profile, were performed. The microscopy images show that the pollen grains of B. pubescens, B. pendula and B. humilis are similar in diameter and significantly smaller than those of others species, with the largest diameter observed for B. utilis Doorenbos. However, the results obtained from FTIR spectroscopy indicate that the chemical compositions of B. pubescens and B. pendula are similar, but B. humilis is outlaying. Summarizing, it is not possible to unambiguously state, which feature or which technique is the best for differentiating between the six chosen birch species. However, the study showed that both techniques have potential for identification of birch pollen species.

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          Most cited references 47

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          An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG II

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            We have developed a quick and low-cost genomic DNA extraction protocol from yeast cells for PCR-based applications. This method does not require any enzymes, hazardous chemicals, or extreme temperatures, and is especially powerful for simultaneous analysis of a large number of samples. DNA can be efficiently extracted from different yeast species (Kluyveromyces lactis, Hansenula polymorpha, Schizosaccharomyces pombe, Candida albicans, Pichia pastoris, and Saccharomyces cerevisiae). The protocol involves lysis of yeast colonies or cells from liquid culture in a lithium acetate (LiOAc)-SDS solution and subsequent precipitation of DNA with ethanol. Approximately 100 nanograms of total genomic DNA can be extracted from 1 × 10(7) cells. DNA extracted by this method is suitable for a variety of PCR-based applications (including colony PCR, real-time qPCR, and DNA sequencing) for amplification of DNA fragments of ≤ 3500 bp.
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              Betula Pendula Roth (B. Verrucosa Ehrh.) and B. Pubescens Ehrh.

               M Atkinson (1992)

                Author and article information

                Aerobiologia (Bologna)
                Aerobiologia (Bologna)
                Springer Netherlands (Dordrecht )
                4 July 2018
                4 July 2018
                : 34
                : 4
                : 525-538
                [1 ]ISNI 0000 0001 0942 8941, GRID grid.418860.3, Institute of Nuclear Physics Polish Academy of Sciences, ; 31342 Kraków, Poland
                [2 ]ISNI 0000 0001 2154 3176, GRID grid.13856.39, Department of Ecology and Environmental Biology, , University of Rzeszow, ; Zelwerowicza 4, 35-601 Rzeszow, Poland
                © The Author(s) 2018

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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                © Springer Nature B.V. 2018


                sem, birch, ftir spectroscopy, pollen, palynotaxonomy, betula


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