BCAT1 is a New MR Imaging-related Biomarker for Prognosis Prediction in IDH1-wildtype Glioblastoma Patients

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Abstract

Isocitrate dehydrogenase 1 (IDH1)-wildtype glioblastoma (GBM) has found to be accompanied with increased expression of branched-chain amino acid trasaminase1 (BCAT1), which is associated with tumor growth and disease progression. In this retrospective study, quantitative RT-PCR, immunohistochemistry, and western blot were performed with GBM patient tissues to evaluate the BCAT1 level. Quantitative MR imaging parameters were evaluated from DSC perfusion imaging, DWI, contrast-enhanced T1WI and FLAIR imaging using a 3T MR scanner. The level of BCAT1 was significantly higher in IDH1-wildtype patients than in IDH1-mutant patients obtained in immunohistochemistry and western blot. The BCAT1 level was significantly correlated with the mean and 95 ^th percentile-normalized CBV as well as the mean ADC based on FLAIR images. In addition, the 95 ^th percentile-normalized CBV from CE T1WI also had a significant correlation with the BCAT1 level. Moreover, the median PFS in patients with BCAT1 expression <100 was longer than in those with BCAT1 expression ≥100. Taken together, we found that a high BCAT1 level is correlated with high CBV and a low ADC value as well as the poor prognosis of BCAT1 expression is related to the aggressive nature of GBM.

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Author and article information

Contributors

Seung Hong Choi:

ORCID: http://orcid.org/0000-0002-0412-2270

verocay@snuh.org

Journal

Journal ID (nlm-ta): Sci Rep

Journal ID (iso-abbrev): Sci Rep

Title: Scientific Reports

Publisher: Nature Publishing Group UK (London )

ISSN (Electronic): 2045-2322

Publication date (Electronic): 18 December 2017

Publication date PMC-release: 18 December 2017

Publication date Collection: 2017

Volume: 7

Affiliations

[1 ]ISNI 0000 0001 0302 820X, GRID grid.412484.f, Department of Radiology, Seoul National University Hospital, ; Seoul, Korea

[2 ]ISNI 0000 0004 1784 4496, GRID grid.410720.0, Center for Nanoparticle Research, Institute for Basic Science (IBS), ; Seoul, Korea

[3 ]ISNI 0000 0001 0302 820X, GRID grid.412484.f, Department of Neurosurgery, Seoul National University Hospital, ; Seoul, Korea

[4 ]ISNI 0000 0001 0302 820X, GRID grid.412484.f, Department of Pathology, Seoul National University Hospital, ; Seoul, Korea

Article

Publisher ID: 17062

DOI: 10.1038/s41598-017-17062-1

PMC ID: 5735129

PubMed ID: 29255149

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

BCAT1 is a New MR Imaging-related Biomarker for Prognosis Prediction in IDH1-wildtype Glioblastoma Patients

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Most cited references 27

High resolution measurement of cerebral blood flow using intravascular tracer bolus passages. Part I: Mathematical approach and statistical analysis.

Identification of noninvasive imaging surrogates for brain tumor gene-expression modules.

MR imaging predictors of molecular profile and survival: multi-institutional study of the TCGA glioblastoma data set.

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