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      Micropropagação de porta-enxertos híbridos de Vitis labrusca x Vitis rotundifolia com resistência à pérola-da-terra (Eurhizococcus brasiliensis Hempel, Hemiptera: Margarodidae) Translated title: Micro propagation of hybrids of Vitis labrusca x Vitis rotundifolia rootstocks with resistance to perola-da-terra (Eurhizococcus brasiliensis Hempel, Hemiptera: Margarodidae)

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          Abstract

          A produção homogênea de porta-enxertos resistentes à pérola-da-terra (Eurhizococcus brasiliensis Hempel, Hemiptera: Margarodidae) e adaptados à região vitivinícola do Sul do País, pela técnica de micropropagação, poderá vir a atender à demanda do setor produtivo de uva e evitar as perdas causadas pela praga. O objetivo deste trabalho foi desenvolver um protocolo de micropropagação dos híbridos 1 e 2, que possuem as qualidades genéticas e de vigor desejadas para porta-enxerto, e resistência à pérola-da-terra. A partir de gemas axilares cultivadas em meio Galzy adicionado de 3µM de bezilaminopurina (BAP), foi possível induzir a multibrotação, com um número satisfatório de brotos, passível de ser incrementado por subcultivos sucessivos no mesmo meio de cultura. Os brotos obtidos neste meio e transferidos para o meio de cultura Galzy, adicionado de 8µM.10-3 de ácido naftalenoacético (ANA), enraizaram em 100%, superando a dificuldade de enraizamento que em geral tem sido a maior barreira apresentada pela espécie V. rotundifolia e seus híbridos.

          Translated abstract

          The homogeneous production of rootstock resistant to perola-da-terra (Eurhizococcus brasiliensis Hempel, Hemiptera: Margarodidae) and well adapted to the growth conditions of the main grape growing region of Southern Brazil by the use of the micropropagation technique, may come to solve the demand of the grape productive sector and avoid losses caused by this pest. The objective of this work was to develop a protocol of micro propagation for the hybrids 1 and 2, which have the desired genetic qualities and vigor to be used as rootstock resistant to perola-da-terra. From axillaries buds cultivated in Galzy culture medium with 3µM of BAP, it was possible to induce multiple shooting with a reasonable number of shoots, which may be improved by successive subcultures in the same medium. The shoots were transferred to Galzy culture medium with 8µM.10-3 of NAA and all of them rooted, overcoming the rooting step that has been pointed out as the main barrier for micro propagation of the species Viits rotundifolia and its hybrids.

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          Most cited references20

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          The potential role of (vinifera x rotundifolia) hybrids in grape variety improvement

          H. Olmo (1986)
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            In vitro assessment of grapevine resistance to two populations of phylloxera from Australian vineyards

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              Technique de thermotherapie des viroses de la vigne

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rbf
                Revista Brasileira de Fruticultura
                Rev. Bras. Frutic.
                Sociedade Brasileira de Fruticultura (Jaboticabal )
                1806-9967
                August 2007
                : 29
                : 2
                : 350-354
                Affiliations
                [1 ] Embrapa Tabuleiros Costeiros Brazil
                [2 ] Universidade Federal do Rio Grande do Sul Brazil
                [3 ] Embrapa Uva e Vinho Brazil
                [4 ] Embrapa Uva e Vinho Brazil
                Article
                S0100-29452007000200031
                10.1590/S0100-29452007000200031
                f39cfa33-da3e-4796-8e0a-c984ef09f28b

                http://creativecommons.org/licenses/by/4.0/

                History
                Product

                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=0100-2945&lng=en
                Categories
                HORTICULTURE

                Horticulture
                Cultivo in vitro,grapevine,rootstocks,muscadine grape,videira,uvas muscadíneas
                Horticulture
                Cultivo in vitro, grapevine, rootstocks, muscadine grape, videira, uvas muscadíneas

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